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Recombinant Simian Varicella Virus-Simian Immunodeficiency Virus Vaccine Induces T and B Cell Functions and Provides Partial Protection against Repeated Mucosal SIV Challenges in Rhesus Macaques.

Publication ,  Journal Article
Pahar, B; Gray, W; Fahlberg, M; Grasperge, B; Hunter, M; Das, A; Mabee, C; Aye, PP; Schiro, F; Hensley, K; Ratnayake, A; Goff, K; Shen, X ...
Published in: Viruses
December 17, 2022

HIV vaccine mediated efficacy, using an expanded live attenuated recombinant varicella virus-vectored SIV rSVV-SIVgag/env vaccine prime with adjuvanted SIV-Env and SIV-Gag protein boosts, was evaluated in a female rhesus macaques (RM) model against repeated intravaginal SIV challenges. Vaccination induced anti-SIV IgG responses and neutralizing antibodies were found in all vaccinated RMs. Three of the eight vaccinated RM remained uninfected (vaccinated and protected, VP) after 13 repeated challenges with the pathogenic SIVmac251-CX-1. The remaining five vaccinated and infected (VI) macaques had significantly reduced plasma viral loads compared with the infected controls (IC). A significant increase in systemic central memory CD4+ T cells and mucosal CD8+ effector memory T-cell responses was detected in vaccinated RMs compared to controls. Variability in lymph node SIV-Gag and Env specific CD4+ and CD8+ T cell cytokine responses were detected in the VI RMs while all three VP RMs had more durable cytokine responses following vaccination and prior to challenge. VI RMs demonstrated predominately SIV-specific monofunctional cytokine responses while the VP RMs generated polyfunctional cytokine responses. This study demonstrates that varicella virus-vectored SIV vaccination with protein boosts induces a 37.5% efficacy rate against pathogenic SIV challenge by generating mucosal memory, virus specific neutralizing antibodies, binding antibodies, and polyfunctional T-cell responses.

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Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

December 17, 2022

Volume

14

Issue

12

Location

Switzerland

Related Subject Headings

  • Vaccines, Synthetic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Macaca mulatta
  • Female
  • Cytokines
  • Chickenpox
  • Antibodies, Viral
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Pahar, B., Gray, W., Fahlberg, M., Grasperge, B., Hunter, M., Das, A., … Traina-Dorge, V. (2022). Recombinant Simian Varicella Virus-Simian Immunodeficiency Virus Vaccine Induces T and B Cell Functions and Provides Partial Protection against Repeated Mucosal SIV Challenges in Rhesus Macaques. Viruses, 14(12). https://doi.org/10.3390/v14122819
Pahar, Bapi, Wayne Gray, Marissa Fahlberg, Brooke Grasperge, Meredith Hunter, Arpita Das, Christopher Mabee, et al. “Recombinant Simian Varicella Virus-Simian Immunodeficiency Virus Vaccine Induces T and B Cell Functions and Provides Partial Protection against Repeated Mucosal SIV Challenges in Rhesus Macaques.Viruses 14, no. 12 (December 17, 2022). https://doi.org/10.3390/v14122819.
Pahar B, Gray W, Fahlberg M, Grasperge B, Hunter M, Das A, Mabee C, Aye PP, Schiro F, Hensley K, Ratnayake A, Goff K, LaBranche C, Shen X, Tomaras GD, DeMarco CT, Montefiori D, Kissinger P, Marx PA, Traina-Dorge V. Recombinant Simian Varicella Virus-Simian Immunodeficiency Virus Vaccine Induces T and B Cell Functions and Provides Partial Protection against Repeated Mucosal SIV Challenges in Rhesus Macaques. Viruses. 2022 Dec 17;14(12).

Published In

Viruses

DOI

EISSN

1999-4915

Publication Date

December 17, 2022

Volume

14

Issue

12

Location

Switzerland

Related Subject Headings

  • Vaccines, Synthetic
  • Simian immunodeficiency virus
  • Simian Immunodeficiency Virus
  • Simian Acquired Immunodeficiency Syndrome
  • SAIDS Vaccines
  • Macaca mulatta
  • Female
  • Cytokines
  • Chickenpox
  • Antibodies, Viral