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Progesterone Signaling in Endometrial Epithelial Organoids.

Publication ,  Journal Article
Hewitt, SC; Wu, S-P; Wang, T; Young, SL; Spencer, TE; DeMayo, FJ
Published in: Cells
May 27, 2022

For pregnancy to be established, uterine cells respond to the ovarian hormones, estrogen, and progesterone, via their nuclear receptors, the estrogen receptor (ESR1) and progesterone receptor (PGR). ESR1 and PGR regulate genes by binding chromatin at genes and at distal enhancer regions, which interact via dynamic 3-dimensional chromatin structures. Endometrial epithelial cells are the initial site of embryo attachment and invasion, and thus understanding the processes that yield their receptive state is important. Here, we cultured and treated organoids derived from human epithelial cells, isolated from endometrial biopsies, with estrogen and progesterone and evaluated their transcriptional profiles, their PGR cistrome, and their chromatin conformation. Progesterone attenuated estrogen-dependent gene responses but otherwise minimally impacted the organoid transcriptome. PGR ChIPseq peaks were co-localized with previously described organoid ESR1 peaks, and most PGR and ESR1 peaks were in B (inactive) compartment regions of chromatin. Significantly more ESR1 peaks were assigned to estrogen-regulated genes by considering chromatin loops identified using HiC than were identified using ESR1 peak location relative to closest genes. Overall, the organoids model allowed a definition of the chromatin regulatory components governing hormone responsiveness.

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Published In

Cells

DOI

EISSN

2073-4409

Publication Date

May 27, 2022

Volume

11

Issue

11

Location

Switzerland

Related Subject Headings

  • Receptors, Estrogen
  • Progesterone
  • Pregnancy
  • Organoids
  • Humans
  • Female
  • Estrogens
  • Endometrium
  • Chromatin
  • 32 Biomedical and clinical sciences
 

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Hewitt, S. C., Wu, S.-P., Wang, T., Young, S. L., Spencer, T. E., & DeMayo, F. J. (2022). Progesterone Signaling in Endometrial Epithelial Organoids. Cells, 11(11). https://doi.org/10.3390/cells11111760
Hewitt, Sylvia C., San-Pin Wu, Tianyuan Wang, Steven L. Young, Thomas E. Spencer, and Francesco J. DeMayo. “Progesterone Signaling in Endometrial Epithelial Organoids.Cells 11, no. 11 (May 27, 2022). https://doi.org/10.3390/cells11111760.
Hewitt SC, Wu S-P, Wang T, Young SL, Spencer TE, DeMayo FJ. Progesterone Signaling in Endometrial Epithelial Organoids. Cells. 2022 May 27;11(11).
Hewitt, Sylvia C., et al. “Progesterone Signaling in Endometrial Epithelial Organoids.Cells, vol. 11, no. 11, May 2022. Pubmed, doi:10.3390/cells11111760.
Hewitt SC, Wu S-P, Wang T, Young SL, Spencer TE, DeMayo FJ. Progesterone Signaling in Endometrial Epithelial Organoids. Cells. 2022 May 27;11(11).

Published In

Cells

DOI

EISSN

2073-4409

Publication Date

May 27, 2022

Volume

11

Issue

11

Location

Switzerland

Related Subject Headings

  • Receptors, Estrogen
  • Progesterone
  • Pregnancy
  • Organoids
  • Humans
  • Female
  • Estrogens
  • Endometrium
  • Chromatin
  • 32 Biomedical and clinical sciences