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G protein-coupled estrogen receptor (GPER) expression in normal and abnormal endometrium.

Publication ,  Journal Article
Plante, BJ; Lessey, BA; Taylor, RN; Wang, W; Bagchi, MK; Yuan, L; Scotchie, J; Fritz, MA; Young, SL
Published in: Reprod Sci
July 2012

Rapid estrogen effects are mediated by membrane receptors, and evidence suggests a role for both a membrane-associated form of estrogen receptor alpha (ESR1; ERα) and G-protein coupled receptor 30 (GPER; GPR30). Considering estrogen's importance in endometrial physiology and endometriosis pathophysiology, we hypothesized that GPER could be involved in both cyclic changes in endometrial estrogen action and that aberrant expression might be seen in the eutopic endometrium of women with endometriosis. Using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemical analysis of normal endometrium, endometrial samples demonstrated cycle-regulated expression of GPER, with maximal expression in the proliferative phase. Eutopic and ectopic endometrium from women with endometriosis overexpressed GPER as compared to eutopic endometrium of normal participants. Ishikawa cells, an adenocarcinoma cell line, expressed GPER, with increased expression upon treatment with estrogen or an ESR1 agonist, but not with a GPER-specific agonist. Decreased expression was seen in Ishikawa cells stably transfected with progesterone receptor A. Together, these data suggest that normal endometrial GPER expression is cyclic and regulated by nuclear estrogen and progesterone receptors, while expression is dysregulated in endometriosis.

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Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

July 2012

Volume

19

Issue

7

Start / End Page

684 / 693

Location

United States

Related Subject Headings

  • Young Adult
  • Tissue Banks
  • Stromal Cells
  • Retrospective Studies
  • Receptors, G-Protein-Coupled
  • Receptors, Estrogen
  • RNA, Messenger
  • Obstetrics & Reproductive Medicine
  • Menstrual Cycle
  • Humans
 

Citation

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Plante, B. J., Lessey, B. A., Taylor, R. N., Wang, W., Bagchi, M. K., Yuan, L., … Young, S. L. (2012). G protein-coupled estrogen receptor (GPER) expression in normal and abnormal endometrium. Reprod Sci, 19(7), 684–693. https://doi.org/10.1177/1933719111431000
Plante, Beth J., Bruce A. Lessey, Robert N. Taylor, Wei Wang, Milan K. Bagchi, Lingwen Yuan, Jessica Scotchie, Marc A. Fritz, and Steven L. Young. “G protein-coupled estrogen receptor (GPER) expression in normal and abnormal endometrium.Reprod Sci 19, no. 7 (July 2012): 684–93. https://doi.org/10.1177/1933719111431000.
Plante BJ, Lessey BA, Taylor RN, Wang W, Bagchi MK, Yuan L, et al. G protein-coupled estrogen receptor (GPER) expression in normal and abnormal endometrium. Reprod Sci. 2012 Jul;19(7):684–93.
Plante, Beth J., et al. “G protein-coupled estrogen receptor (GPER) expression in normal and abnormal endometrium.Reprod Sci, vol. 19, no. 7, July 2012, pp. 684–93. Pubmed, doi:10.1177/1933719111431000.
Plante BJ, Lessey BA, Taylor RN, Wang W, Bagchi MK, Yuan L, Scotchie J, Fritz MA, Young SL. G protein-coupled estrogen receptor (GPER) expression in normal and abnormal endometrium. Reprod Sci. 2012 Jul;19(7):684–693.
Journal cover image

Published In

Reprod Sci

DOI

EISSN

1933-7205

Publication Date

July 2012

Volume

19

Issue

7

Start / End Page

684 / 693

Location

United States

Related Subject Headings

  • Young Adult
  • Tissue Banks
  • Stromal Cells
  • Retrospective Studies
  • Receptors, G-Protein-Coupled
  • Receptors, Estrogen
  • RNA, Messenger
  • Obstetrics & Reproductive Medicine
  • Menstrual Cycle
  • Humans