Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2.
Publication
, Journal Article
Hsueh, Y-C; Pratt, RE; Dzau, VJ; Hodgkinson, CP
Published in: Sci Rep
March 9, 2023
Current methods to generate cardiomyocytes from induced pluripotent stem cells (iPSc) utilize broad-spectrum pharmacological inhibitors. These methods give rise to cardiomyocytes which are typically immature. Since we have recently demonstrated that cardiomyogenesis in vitro and in vivo requires Sfrp2, we asked if Sfrp2 would drive differentiation of human iPSc into cardiomyocytes. Indeed, we found that Sfrp2 induced robust cardiac differentiation. Importantly, replacement of broad spectrum pharmacological inhibitors with Sfrp2 gave rise to mature cardiomyocytes as evidenced by their sarcomere structure, electrophysiological profiles, and ability to form gap junctions.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
Sci Rep
DOI
EISSN
2045-2322
Publication Date
March 9, 2023
Volume
13
Issue
1
Start / End Page
3920
Location
England
Related Subject Headings
- Myocytes, Cardiac
- Membrane Proteins
- Induced Pluripotent Stem Cells
- Humans
- Electrophysiological Phenomena
- Cell Differentiation
Citation
APA
Chicago
ICMJE
MLA
NLM
Hsueh, Y.-C., Pratt, R. E., Dzau, V. J., & Hodgkinson, C. P. (2023). Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Sci Rep, 13(1), 3920. https://doi.org/10.1038/s41598-023-31144-3
Hsueh, Ying-Chang, Richard E. Pratt, Victor J. Dzau, and Conrad P. Hodgkinson. “Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2.” Sci Rep 13, no. 1 (March 9, 2023): 3920. https://doi.org/10.1038/s41598-023-31144-3.
Hsueh Y-C, Pratt RE, Dzau VJ, Hodgkinson CP. Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Sci Rep. 2023 Mar 9;13(1):3920.
Hsueh, Ying-Chang, et al. “Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2.” Sci Rep, vol. 13, no. 1, Mar. 2023, p. 3920. Pubmed, doi:10.1038/s41598-023-31144-3.
Hsueh Y-C, Pratt RE, Dzau VJ, Hodgkinson CP. Novel method of differentiating human induced pluripotent stem cells to mature cardiomyocytes via Sfrp2. Sci Rep. 2023 Mar 9;13(1):3920.
Published In
Sci Rep
DOI
EISSN
2045-2322
Publication Date
March 9, 2023
Volume
13
Issue
1
Start / End Page
3920
Location
England
Related Subject Headings
- Myocytes, Cardiac
- Membrane Proteins
- Induced Pluripotent Stem Cells
- Humans
- Electrophysiological Phenomena
- Cell Differentiation