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Digital spatial profiling of intraductal papillary mucinous neoplasms: Toward a molecular framework for risk stratification.

Publication ,  Journal Article
Iyer, MK; Shi, C; Eckhoff, AM; Fletcher, A; Nussbaum, DP; Allen, PJ
Published in: Sci Adv
March 15, 2023

The histopathologic heterogeneity of intraductal papillary mucinous neoplasms (IPMN) complicates the prediction of pancreatic ductal adenocarcinoma (PDAC) risk. Intratumoral regions of pancreaticobiliary (PB), intestinal (INT), and gastric foveolar (GF) epithelium may occur with either low-grade dysplasia (LGD) or high-grade dysplasia (HGD). We used digital spatial RNA profiling of dysplastic epithelium (83 regions) from surgically resected IPMN tissues (12 patients) to differentiate subtypes and predict genes associated with malignancy. The expression patterns of PB and GF lesions diverged from INT, suggesting that PB and GF arise from a common lineage. Transcriptional dysregulation within PB lesions mirrored that of PDAC, whereas INT and GF foci did not. Tumor necrosis factor/nuclear factor κB (TNF-NFκB) and cell cycle (cycling S and cycling G2-M) programs occurred with relative prominence in PB and INT subtypes, respectively. Together, this study delineates markers of high-risk IPMN and insights into malignant progression.

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Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

March 15, 2023

Volume

9

Issue

11

Start / End Page

eade4582

Location

United States

Related Subject Headings

  • Risk Assessment
  • Pancreatic Neoplasms
  • Pancreatic Intraductal Neoplasms
  • Neoplasms, Cystic, Mucinous, and Serous
  • Humans
  • Carcinoma, Pancreatic Ductal
 

Citation

APA
Chicago
ICMJE
MLA
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Iyer, M. K., Shi, C., Eckhoff, A. M., Fletcher, A., Nussbaum, D. P., & Allen, P. J. (2023). Digital spatial profiling of intraductal papillary mucinous neoplasms: Toward a molecular framework for risk stratification. Sci Adv, 9(11), eade4582. https://doi.org/10.1126/sciadv.ade4582
Iyer, Matthew K., Chanjuan Shi, Austin M. Eckhoff, Ashley Fletcher, Daniel P. Nussbaum, and Peter J. Allen. “Digital spatial profiling of intraductal papillary mucinous neoplasms: Toward a molecular framework for risk stratification.Sci Adv 9, no. 11 (March 15, 2023): eade4582. https://doi.org/10.1126/sciadv.ade4582.
Iyer MK, Shi C, Eckhoff AM, Fletcher A, Nussbaum DP, Allen PJ. Digital spatial profiling of intraductal papillary mucinous neoplasms: Toward a molecular framework for risk stratification. Sci Adv. 2023 Mar 15;9(11):eade4582.
Iyer, Matthew K., et al. “Digital spatial profiling of intraductal papillary mucinous neoplasms: Toward a molecular framework for risk stratification.Sci Adv, vol. 9, no. 11, Mar. 2023, p. eade4582. Pubmed, doi:10.1126/sciadv.ade4582.
Iyer MK, Shi C, Eckhoff AM, Fletcher A, Nussbaum DP, Allen PJ. Digital spatial profiling of intraductal papillary mucinous neoplasms: Toward a molecular framework for risk stratification. Sci Adv. 2023 Mar 15;9(11):eade4582.

Published In

Sci Adv

DOI

EISSN

2375-2548

Publication Date

March 15, 2023

Volume

9

Issue

11

Start / End Page

eade4582

Location

United States

Related Subject Headings

  • Risk Assessment
  • Pancreatic Neoplasms
  • Pancreatic Intraductal Neoplasms
  • Neoplasms, Cystic, Mucinous, and Serous
  • Humans
  • Carcinoma, Pancreatic Ductal