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Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses.

Publication ,  Journal Article
Khadka, S; Omura, S; Sato, F; Tsunoda, I
Published in: International journal of molecular sciences
February 2023

Alterations in the gut microbiota, "dysbiosis," have been reported in autoimmune diseases, including multiple sclerosis (MS), and their animal models. Although the animal models were induced by injections of autoantigens with adjuvants, including complete Freund's adjuvant (CFA) and pertussis toxin (PT), the effects of adjuvant injections on the microbiota are largely unknown. We aimed to clarify whether adjuvant injections could affect the microbiota in the ileum and feces. Using 16S rRNA sequencing, we found decreased alpha diversities of the gut microbiota in mice injected with CFA and PT, compared with naïve mice. Overall, microbial profiles visualized by principal component analysis demonstrated dysbiosis in feces, but not in the ileum, of adjuvant-injected mice, where the genera Lachnospiraceae NK4A136 group and Alistipes contributed to dysbiosis. When we compared the relative abundances of individual bacteria, we found changes in 16 bacterial genera in feces and seven genera in the ileum of adjuvant-injected mice, in which increased serum levels of antibody against mycobacteria (a component of CFA) and total IgG2c were correlated with the genus Facklamia. On the other hand, increased IgG1 and IgA concentrations were correlated with the genus Atopostipes. Therefore, adjuvant injections alone could alter the overall microbial profiles (i.e., microbiota) and individual bacterial abundances with altered antibody responses; dysbiosis in animal models could be partly due to adjuvant injections.

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Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

February 2023

Volume

24

Issue

3

Start / End Page

2818

Related Subject Headings

  • RNA, Ribosomal, 16S
  • Mice
  • Immunoglobulin G
  • Ileum
  • Gastrointestinal Microbiome
  • Freund's Adjuvant
  • Feces
  • Dysbiosis
  • Chemical Physics
  • Bacteria
 

Citation

APA
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MLA
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Khadka, S., Omura, S., Sato, F., & Tsunoda, I. (2023). Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses. International Journal of Molecular Sciences, 24(3), 2818. https://doi.org/10.3390/ijms24032818
Khadka, Sundar, Seiichi Omura, Fumitaka Sato, and Ikuo Tsunoda. “Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses.International Journal of Molecular Sciences 24, no. 3 (February 2023): 2818. https://doi.org/10.3390/ijms24032818.
Khadka S, Omura S, Sato F, Tsunoda I. Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses. International journal of molecular sciences. 2023 Feb;24(3):2818.
Khadka, Sundar, et al. “Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses.International Journal of Molecular Sciences, vol. 24, no. 3, Feb. 2023, p. 2818. Epmc, doi:10.3390/ijms24032818.
Khadka S, Omura S, Sato F, Tsunoda I. Adjuvant Injections Altered the Ileal and Fecal Microbiota Differently with Changes in Immunoglobulin Isotypes and Antimycobacterial Antibody Responses. International journal of molecular sciences. 2023 Feb;24(3):2818.

Published In

International journal of molecular sciences

DOI

EISSN

1422-0067

ISSN

1422-0067

Publication Date

February 2023

Volume

24

Issue

3

Start / End Page

2818

Related Subject Headings

  • RNA, Ribosomal, 16S
  • Mice
  • Immunoglobulin G
  • Ileum
  • Gastrointestinal Microbiome
  • Freund's Adjuvant
  • Feces
  • Dysbiosis
  • Chemical Physics
  • Bacteria