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Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging.

Publication ,  Journal Article
Quardokus, EM; Saunders, DC; McDonough, E; Hickey, JW; Werlein, C; Surrette, C; Rajbhandari, P; Casals, AM; Tian, H; Lowery, L; Neumann, EK ...
Published in: Nature methods
August 2023

Multiplexed antibody-based imaging enables the detailed characterization of molecular and cellular organization in tissues. Advances in the field now allow high-parameter data collection (>60 targets); however, considerable expertise and capital are needed to construct the antibody panels employed by these methods. Organ mapping antibody panels are community-validated resources that save time and money, increase reproducibility, accelerate discovery and support the construction of a Human Reference Atlas.

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Published In

Nature methods

DOI

EISSN

1548-7105

ISSN

1548-7091

Publication Date

August 2023

Volume

20

Issue

8

Start / End Page

1174 / 1178

Related Subject Headings

  • Reproducibility of Results
  • Humans
  • Diagnostic Imaging
  • Developmental Biology
  • Community Resources
  • Antibodies
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 10 Technology
  • 06 Biological Sciences
 

Citation

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Quardokus, E. M., Saunders, D. C., McDonough, E., Hickey, J. W., Werlein, C., Surrette, C., … Radtke, A. J. (2023). Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging. Nature Methods, 20(8), 1174–1178. https://doi.org/10.1038/s41592-023-01846-7
Quardokus, Ellen M., Diane C. Saunders, Elizabeth McDonough, John W. Hickey, Christopher Werlein, Christine Surrette, Presha Rajbhandari, et al. “Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging.Nature Methods 20, no. 8 (August 2023): 1174–78. https://doi.org/10.1038/s41592-023-01846-7.
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, et al. Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging. Nature methods. 2023 Aug;20(8):1174–8.
Quardokus, Ellen M., et al. “Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging.Nature Methods, vol. 20, no. 8, Aug. 2023, pp. 1174–78. Epmc, doi:10.1038/s41592-023-01846-7.
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Casals AM, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Muller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ. Organ Mapping Antibody Panels: a community resource for standardized multiplexed tissue imaging. Nature methods. 2023 Aug;20(8):1174–1178.

Published In

Nature methods

DOI

EISSN

1548-7105

ISSN

1548-7091

Publication Date

August 2023

Volume

20

Issue

8

Start / End Page

1174 / 1178

Related Subject Headings

  • Reproducibility of Results
  • Humans
  • Diagnostic Imaging
  • Developmental Biology
  • Community Resources
  • Antibodies
  • 31 Biological sciences
  • 11 Medical and Health Sciences
  • 10 Technology
  • 06 Biological Sciences