Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles.
We have developed a tool to both enrich and expand antigen-specific T cells. This can be helpful in cases such as to A) detect the existence of antigen-specific T cells, B) probe the dynamics of antigen-specific responses, C) understand how antigen-specific responses affect disease state such as autoimmunity, D) demystify heterogeneous responses for antigen-specific T cells, or E) utilize antigen-specific cells for therapy. The tool is based on a magnetic particle that we conjugate antigen-specific and T cell co-stimulatory signals, and that we term as artificial antigen presenting cells (aAPCs). Consequently, since the technology is simple to produce, it can easily be adopted by other laboratories; thus, our purpose here is to describe in detail the fabrication and subsequent use of the aAPCs. We explain how to attach antigen-specific and co-stimulatory signals to the aAPCs, how to utilize them to enrich for antigen-specific T cells, and how to expand antigen-specific T cells. Furthermore, we will highlight engineering design considerations based on experimental and biological information of our experience with characterizing antigen-specific T cells.
Duke Scholars
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Related Subject Headings
- T-Lymphocytes
- Magnetite Nanoparticles
- Humans
- Bioengineering
- Antigen-Presenting Cells
- 3101 Biochemistry and cell biology
- 1702 Cognitive Sciences
- 1701 Psychology
- 0601 Biochemistry and Cell Biology
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Issue
Related Subject Headings
- T-Lymphocytes
- Magnetite Nanoparticles
- Humans
- Bioengineering
- Antigen-Presenting Cells
- 3101 Biochemistry and cell biology
- 1702 Cognitive Sciences
- 1701 Psychology
- 0601 Biochemistry and Cell Biology