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Lymphokine-activated killer cell activity after cryopreservation.

Publication ,  Journal Article
Schmidt-Wolf, IG; Aihara, M; Negrin, RS; Blume, KG; Chao, NJ
Published in: J Immunol Methods
December 20, 1989
Published version (DOI) Link to item

The effect of cryopreservation on the cytotoxic activity of lymphokine-activated killer (LAK) cells was studied. LAK cells were generated by incubating peripheral blood lymphocytes for 3-5 days with recombinant interleukin-2 (rIL-2) and then cryopreserved using a programmed freezer. Cytotoxicity was determined in a 51Cr release assay. After thawing, the LAK cells had reduced cytotoxicity (25.5-39.1% as compared to the original lytic units). Cytotoxic activity could be restored to pre-cryopreserved levels by reincubation with rIL-2 for 2 days after thawing. Thus, maximal cytotoxicity of cryopreserved LAK cells could be achieved by incubation with rIL-2 before and after the freezing process. The level of cytotoxicity was comparable to that of LAK cells from fresh peripheral blood lymphocytes. Cryopreserved LAK cells may have potential in adoptive immunotherapy.

Duke Scholars

Nelson Jen An Chao
Author Nelson Jen An Chao Medicine, Hematologic Malignancies and Cellular Therapy

Published In

J Immunol Methods

DOI

10.1016/0022-1759(89)90092-6

ISSN

0022-1759

Publication Date

December 20, 1989

Volume

125

Issue

1-2

Start / End Page

185 / 189

Location

Netherlands

Related Subject Headings

  • Recombinant Proteins
  • Preservation, Biological
  • Killer Cells, Lymphokine-Activated
  • Interleukin-2
  • In Vitro Techniques
  • Immunology
  • Immunity, Cellular
  • Humans
  • Freezing
  • Cytotoxicity, Immunologic
 

Citation

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ICMJE
MLA
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Schmidt-Wolf, I. G., Aihara, M., Negrin, R. S., Blume, K. G., & Chao, N. J. (1989). Lymphokine-activated killer cell activity after cryopreservation. J Immunol Methods, 125(1–2), 185–189. https://doi.org/10.1016/0022-1759(89)90092-6
Schmidt-Wolf, I. G., M. Aihara, R. S. Negrin, K. G. Blume, and N. J. Chao. “Lymphokine-activated killer cell activity after cryopreservation.” J Immunol Methods 125, no. 1–2 (December 20, 1989): 185–89. https://doi.org/10.1016/0022-1759(89)90092-6.
Schmidt-Wolf IG, Aihara M, Negrin RS, Blume KG, Chao NJ. Lymphokine-activated killer cell activity after cryopreservation. J Immunol Methods. 1989 Dec 20;125(1–2):185–9.
Schmidt-Wolf, I. G., et al. “Lymphokine-activated killer cell activity after cryopreservation.” J Immunol Methods, vol. 125, no. 1–2, Dec. 1989, pp. 185–89. Pubmed, doi:10.1016/0022-1759(89)90092-6.
Schmidt-Wolf IG, Aihara M, Negrin RS, Blume KG, Chao NJ. Lymphokine-activated killer cell activity after cryopreservation. J Immunol Methods. 1989 Dec 20;125(1–2):185–189.
Journal cover image

Published In

J Immunol Methods

DOI

10.1016/0022-1759(89)90092-6

ISSN

0022-1759

Publication Date

December 20, 1989

Volume

125

Issue

1-2

Start / End Page

185 / 189

Location

Netherlands

Related Subject Headings

  • Recombinant Proteins
  • Preservation, Biological
  • Killer Cells, Lymphokine-Activated
  • Interleukin-2
  • In Vitro Techniques
  • Immunology
  • Immunity, Cellular
  • Humans
  • Freezing
  • Cytotoxicity, Immunologic