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Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1.

Publication ,  Journal Article
Jiao, K; Zhou, Y; Hogan, BLM
Published in: Mol Cell Biol
November 2002

To identify novel genes that play critical roles in mediating bone morphogenetic protein (BMP) signal pathways, we performed a yeast two-hybrid screen using Smad1 as bait. A novel mouse Krüppel-type zinc finger protein, mZnf8, was isolated. Interactions between mZnf8 and Smad proteins were further analyzed with various in vitro and in vivo approaches, including mammalian two-hybrid, in vitro glutathione S-transferase pulldown, and copurification assays. Results from functional analysis indicate that mZnf8 is a nuclear transcriptional repressor. Overexpression of mZnf8 represses activity of BMP and transforming growth factor beta (TGF-beta) reporters. Silencing the expression of endogenous mZnf8 with an RNA interference approach caused a significant increase in the expression of one BMP reporter. These results suggest that mZnf8 negatively regulates the TGF-beta/BMP signaling pathway in vivo. Transcription of mZnf8 is ubiquitous in mouse embryos, but high levels are specifically observed in adult mouse testes, with the same cell- and stage-specific transcription pattern as Smad1. Our data support the hypothesis that mZnf8 plays critical roles in mediating BMP signaling during spermatogenesis.

Duke Scholars

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Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

November 2002

Volume

22

Issue

21

Start / End Page

7633 / 7644

Location

United States

Related Subject Headings

  • Two-Hybrid System Techniques
  • Transforming Growth Factor beta
  • Transfection
  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Time Factors
  • Testis
  • Spermatogenesis
  • Smad1 Protein
 

Citation

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Jiao, K., Zhou, Y., & Hogan, B. L. M. (2002). Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1. Mol Cell Biol, 22(21), 7633–7644. https://doi.org/10.1128/MCB.22.21.7633-7644.2002
Jiao, Kai, Yingna Zhou, and Brigid L. M. Hogan. “Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1.Mol Cell Biol 22, no. 21 (November 2002): 7633–44. https://doi.org/10.1128/MCB.22.21.7633-7644.2002.
Jiao, Kai, et al. “Identification of mZnf8, a mouse Krüppel-like transcriptional repressor, as a novel nuclear interaction partner of Smad1.Mol Cell Biol, vol. 22, no. 21, Nov. 2002, pp. 7633–44. Pubmed, doi:10.1128/MCB.22.21.7633-7644.2002.

Published In

Mol Cell Biol

DOI

ISSN

0270-7306

Publication Date

November 2002

Volume

22

Issue

21

Start / End Page

7633 / 7644

Location

United States

Related Subject Headings

  • Two-Hybrid System Techniques
  • Transforming Growth Factor beta
  • Transfection
  • Transcription, Genetic
  • Transcription Factors
  • Trans-Activators
  • Time Factors
  • Testis
  • Spermatogenesis
  • Smad1 Protein