Effect of Steel factor and leukaemia inhibitory factor on murine primordial germ cells in culture.
Despite the importance of germ cells to the survival of species, surprisingly little is known about their embryological origin, proliferation, migration and entry into mitotic arrest or meiosis. Mutations in the murine Dominant White Spotting (W) and Steel genes, which respectively encode the c-kit tyrosine kinase receptor and the c-kit ligand (or Steel factor), impair the development of primordial germ cells (PGCs) in vivo, as well as haematopoietic stem cells and neural crest-derived melanoblasts. Here we use a monoclonal antibody against c-kit tyrosine kinase receptor and recombinant Steel factor to study the c-kit receptor-ligand system in cultured PGCs. In addition, we show that leukaemia inhibitory factor (also known as differentiation inhibitory activity), a factor secreted by STO fibroblasts, can stimulate proliferation of primordial germ cells in vitro.
Duke Scholars
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- Proto-Oncogene Proteins c-kit
- Proto-Oncogene Proteins
- Protein-Tyrosine Kinases
- Mice, Inbred ICR
- Mice, Inbred DBA
- Mice, Inbred C57BL
- Mice
- Lymphokines
- Leukemia Inhibitory Factor
- Interleukin-6
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Proto-Oncogene Proteins c-kit
- Proto-Oncogene Proteins
- Protein-Tyrosine Kinases
- Mice, Inbred ICR
- Mice, Inbred DBA
- Mice, Inbred C57BL
- Mice
- Lymphokines
- Leukemia Inhibitory Factor
- Interleukin-6