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Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11.

Publication ,  Journal Article
Mason, IJ; Murphy, D; Münke, M; Francke, U; Elliott, RW; Hogan, BL
Published in: EMBO J
August 1986

SPARC is a Mr 43,000 secreted, acidic, cysteine-rich glycoprotein homologous to 43K bovine endothelial 'culture shock' protein. We show here that it is encoded by a single gene localized to the central region of mouse chromosome 11. During development SPARC mRNA is expressed at higher levels in all the extra-embryonic tissues than in the fetus. Highest levels are found in the parietal endoderm, while visceral endoderm has approximately 6-fold less. This differential expression is also seen in F9 teratocarcinoma cells treated with retinoic acid under conditions in which they give rise to either parietal or visceral endoderm. The 20-fold increase seen during differentiation into parietal endoderm is due, at least in part, to an increase in gene transcription. We also report SPARC expression in a variety of adult tissues and cultured cells, and present evidence that a decrease accompanies the transformation of fibroblast cell lines.

Duke Scholars

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Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

August 1986

Volume

5

Issue

8

Start / End Page

1831 / 1837

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Teratoma
  • RNA, Messenger
  • Pregnancy
  • Osteonectin
  • Nucleic Acid Hybridization
  • Mice, Inbred CBA
  • Mice, Inbred C3H
  • Mice
  • Hybrid Cells
 

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Mason, I. J., Murphy, D., Münke, M., Francke, U., Elliott, R. W., & Hogan, B. L. (1986). Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11. EMBO J, 5(8), 1831–1837. https://doi.org/10.1002/j.1460-2075.1986.tb04434.x
Mason, I. J., D. Murphy, M. Münke, U. Francke, R. W. Elliott, and B. L. Hogan. “Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11.EMBO J 5, no. 8 (August 1986): 1831–37. https://doi.org/10.1002/j.1460-2075.1986.tb04434.x.
Mason IJ, Murphy D, Münke M, Francke U, Elliott RW, Hogan BL. Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11. EMBO J. 1986 Aug;5(8):1831–7.
Mason, I. J., et al. “Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11.EMBO J, vol. 5, no. 8, Aug. 1986, pp. 1831–37. Pubmed, doi:10.1002/j.1460-2075.1986.tb04434.x.
Mason IJ, Murphy D, Münke M, Francke U, Elliott RW, Hogan BL. Developmental and transformation-sensitive expression of the Sparc gene on mouse chromosome 11. EMBO J. 1986 Aug;5(8):1831–1837.

Published In

EMBO J

DOI

ISSN

0261-4189

Publication Date

August 1986

Volume

5

Issue

8

Start / End Page

1831 / 1837

Location

England

Related Subject Headings

  • Transcription, Genetic
  • Teratoma
  • RNA, Messenger
  • Pregnancy
  • Osteonectin
  • Nucleic Acid Hybridization
  • Mice, Inbred CBA
  • Mice, Inbred C3H
  • Mice
  • Hybrid Cells