Skip to main content
Journal cover image

Studies of the ethanol-induced interdigitated gel phase in phosphatidylcholines using the fluorophore 1,6-diphenyl-1,3,5-hexatriene.

Publication ,  Journal Article
Nambi, P; Rowe, ES; McIntosh, TJ
Published in: Biochemistry
December 27, 1988

It is now well established that a number of amphiphilic molecules such as ethanol can induce the formation of the fully interdigitated gel phase in phosphatidylcholines. We have shown earlier that alcohols such as ethanol induce biphasic melting behavior in phosphatidylcholines [Rowe, E. S. (1983) Biochemistry 22, 3299-3305] but not in phosphatidylethanolamines [Rowe, E. S. (1985) Biochim. Biophys. Acta 813, 321-330]. Simon and McIntosh [(1984) Biochim. Biophys. Acta 773, 169-172] showed that the alcohol-induced biphasic melting behavior in phosphatidylcholines is a consequence of acyl chain interdigitation. In the present study we demonstrate the detection of the transition of DPPC and DSPC to the interdigitated phase in the presence of ethanol using the fluorescence properties of the commonly used fluorophore 1,6-diphenyl-1,3,5-hexatriene (DPH). By correlating fluorescence and X-ray diffraction results, we have demonstrated the use of fluorescence to study the phase transition from the noninterdigitated to the interdigitated phase. Using this method, we have investigated the temperature and ethanol concentration dependence of the induction of the interdigitated phase in DSPC and DPPC and shown that the induction of interdigitation by ethanol is temperature dependent, with higher temperature favoring interdigitation. The temperature-ethanol phase diagrams have been determined for DPPC and DSPC.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

December 27, 1988

Volume

27

Issue

26

Start / End Page

9175 / 9182

Location

United States

Related Subject Headings

  • Temperature
  • Phosphatidylcholines
  • Gels
  • Fluorescent Dyes
  • Ethanol
  • Diphenylhexatriene
  • Chemistry, Physical
  • Chemical Phenomena
  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Nambi, P., Rowe, E. S., & McIntosh, T. J. (1988). Studies of the ethanol-induced interdigitated gel phase in phosphatidylcholines using the fluorophore 1,6-diphenyl-1,3,5-hexatriene. Biochemistry, 27(26), 9175–9182. https://doi.org/10.1021/bi00426a015
Nambi, P., E. S. Rowe, and T. J. McIntosh. “Studies of the ethanol-induced interdigitated gel phase in phosphatidylcholines using the fluorophore 1,6-diphenyl-1,3,5-hexatriene.Biochemistry 27, no. 26 (December 27, 1988): 9175–82. https://doi.org/10.1021/bi00426a015.
Nambi, P., et al. “Studies of the ethanol-induced interdigitated gel phase in phosphatidylcholines using the fluorophore 1,6-diphenyl-1,3,5-hexatriene.Biochemistry, vol. 27, no. 26, Dec. 1988, pp. 9175–82. Pubmed, doi:10.1021/bi00426a015.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

December 27, 1988

Volume

27

Issue

26

Start / End Page

9175 / 9182

Location

United States

Related Subject Headings

  • Temperature
  • Phosphatidylcholines
  • Gels
  • Fluorescent Dyes
  • Ethanol
  • Diphenylhexatriene
  • Chemistry, Physical
  • Chemical Phenomena
  • Biochemistry & Molecular Biology
  • 3404 Medicinal and biomolecular chemistry