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Calmodulin is intrinsically LESS effective than troponin C in activating skeletal muscle contraction.

Publication ,  Journal Article
Brandt, PW; George, SE; Schachat, F
Published in: FEBS Lett
October 10, 1994

Calmodulin (CaM) and troponin C (TnC) are evolutionarily and structurally homologous, yet they are not functionally interchangeable. In particular, CaM cannot effectively substitute for TnC as an activator of skeletal muscle contraction. To determine if this is a consequence of CaM's weak association with troponin T and I or the result of a more fundamental mechanistic defect, we have used CaM and a CaM[TnC] chimera, CaM[3,4 TnC], that stably associates with the thin filament. Replacement of TnC with CaM or CaM[3,4 TnC] reveals that CaM-like molecules reduce the Ca(2+)-sensitivity and cooperativity of activation, as well as the maximal Ca(2+)-activated tension. These observations indicate that CaM-like molecules are unable to continuously maintain the activated state of the thin filament.

Duke Scholars

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

October 10, 1994

Volume

353

Issue

1

Start / End Page

99 / 102

Location

England

Related Subject Headings

  • Troponin C
  • Troponin
  • Recombinant Fusion Proteins
  • Rabbits
  • Muscle, Skeletal
  • Muscle Contraction
  • Calmodulin
  • Biochemistry & Molecular Biology
  • Animals
  • 3101 Biochemistry and cell biology
 

Citation

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Brandt, P. W., George, S. E., & Schachat, F. (1994). Calmodulin is intrinsically LESS effective than troponin C in activating skeletal muscle contraction. FEBS Lett, 353(1), 99–102. https://doi.org/10.1016/0014-5793(94)01016-1
Brandt, P. W., S. E. George, and F. Schachat. “Calmodulin is intrinsically LESS effective than troponin C in activating skeletal muscle contraction.FEBS Lett 353, no. 1 (October 10, 1994): 99–102. https://doi.org/10.1016/0014-5793(94)01016-1.
Brandt PW, George SE, Schachat F. Calmodulin is intrinsically LESS effective than troponin C in activating skeletal muscle contraction. FEBS Lett. 1994 Oct 10;353(1):99–102.
Brandt, P. W., et al. “Calmodulin is intrinsically LESS effective than troponin C in activating skeletal muscle contraction.FEBS Lett, vol. 353, no. 1, Oct. 1994, pp. 99–102. Pubmed, doi:10.1016/0014-5793(94)01016-1.
Brandt PW, George SE, Schachat F. Calmodulin is intrinsically LESS effective than troponin C in activating skeletal muscle contraction. FEBS Lett. 1994 Oct 10;353(1):99–102.
Journal cover image

Published In

FEBS Lett

DOI

ISSN

0014-5793

Publication Date

October 10, 1994

Volume

353

Issue

1

Start / End Page

99 / 102

Location

England

Related Subject Headings

  • Troponin C
  • Troponin
  • Recombinant Fusion Proteins
  • Rabbits
  • Muscle, Skeletal
  • Muscle Contraction
  • Calmodulin
  • Biochemistry & Molecular Biology
  • Animals
  • 3101 Biochemistry and cell biology