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Postmitotic expression of ankyrinR and beta R-spectrin in discrete neuronal populations of the rat brain.

Publication ,  Journal Article
Lambert, S; Bennett, V
Published in: J Neurosci
September 1993

Isoforms of ankyrin (ankyrinR) are expressed in both the erythrocyte and the brain. Four cDNAs representing regulatory domains of ankyrinR expressed in the rat spleen and brain were cloned and sequenced. These different cDNAs were found to result from tissue-specific alternative mRNA processing of the ankyrinR regulatory domain. One of the isolated cDNAs was used to develop an antibody to brain isoforms of ankyrinR, and this antibody was used to study the localization of ankyrinR in the rat brain. The protein was found to be widely expressed in neurons of the metencephalon but limited to a discrete subset of neurons in the rat forebrain. In the thalamus and areas of the basal ganglia, these neurons were grouped in defined nuclei, whereas in the cortex, hippocampus, and caudate putamen they appeared as isolated cells distributed randomly throughout these structures. A similar study using an antibody raised against erythrocyte spectrin (beta R) showed a comparable localization to that of ankyrinR. Both proteins were expressed late in the developing rat brain, as part of the maturation stage of neural development. These data suggest a specific role for these erythrocyte structural proteins in the postmitotic development of a subset of neurons in the rat brain.

Duke Scholars

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

September 1993

Volume

13

Issue

9

Start / End Page

3725 / 3735

Location

United States

Related Subject Headings

  • Thalamus
  • Spleen
  • Spectrin
  • Rhombencephalon
  • Regulatory Sequences, Nucleic Acid
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Putamen
  • Prosencephalon
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Lambert, S., & Bennett, V. (1993). Postmitotic expression of ankyrinR and beta R-spectrin in discrete neuronal populations of the rat brain. J Neurosci, 13(9), 3725–3735. https://doi.org/10.1523/JNEUROSCI.13-09-03725.1993
Lambert, S., and V. Bennett. “Postmitotic expression of ankyrinR and beta R-spectrin in discrete neuronal populations of the rat brain.J Neurosci 13, no. 9 (September 1993): 3725–35. https://doi.org/10.1523/JNEUROSCI.13-09-03725.1993.
Lambert, S., and V. Bennett. “Postmitotic expression of ankyrinR and beta R-spectrin in discrete neuronal populations of the rat brain.J Neurosci, vol. 13, no. 9, Sept. 1993, pp. 3725–35. Pubmed, doi:10.1523/JNEUROSCI.13-09-03725.1993.

Published In

J Neurosci

DOI

ISSN

0270-6474

Publication Date

September 1993

Volume

13

Issue

9

Start / End Page

3725 / 3735

Location

United States

Related Subject Headings

  • Thalamus
  • Spleen
  • Spectrin
  • Rhombencephalon
  • Regulatory Sequences, Nucleic Acid
  • Rats, Sprague-Dawley
  • Rats
  • RNA, Messenger
  • Putamen
  • Prosencephalon