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Proteolytic domains of the epidermal growth factor receptor of human placenta.

Publication ,  Journal Article
O'Keefe, EJ; Battin, T; Bennett, V
Published in: J Supramol Struct Cell Biochem
1981

Microsomal membranes form human placenta, which bind 5-20 pmol of 125I-epidermal growth factor (EGF) per mg protein, have been affinity-labeled with 125I-EGF either spontaneously or with dimethylsuberimidate. Coomassie blue staining patterns on SDS polyacrylamide gels are minimally altered, and the EGF-receptor complex appears as a specifically labeled band of 180,000 daltons which is not removed by urea, neutral buffers, or chaotropic salts but is partially extracted by mild detergents. Limited proteolysis by alpha chymotrypsin and several other serine proteases yields labeled fragments of 170,000, 130,000, 85,000, and 48,000 daltons. More facile cleavage by papain or bromelain rapidly degrades the hormone-receptor complex to smaller labeled fragments of about 35,000 and 25,000 daltons. These fragments retain the binding site for EGF, are capable of binding EGF, and remain associated with the membrane. Alpha chymotryptic digestion of receptor solubilized by detergents yields the same fragments obtained with intact vesicles, suggesting that the fragments may represent intrinsic proteolytic domains of the receptor.

Duke Scholars

Published In

J Supramol Struct Cell Biochem

DOI

ISSN

0275-3723

Publication Date

1981

Volume

15

Issue

1

Start / End Page

15 / 27

Location

United States

Related Subject Headings

  • Trypsin
  • Receptors, Cell Surface
  • Pregnancy
  • Placenta
  • Peptides
  • Peptide Fragments
  • Papain
  • Microsomes
  • Kinetics
  • Intracellular Membranes
 

Citation

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O’Keefe, E. J., Battin, T., & Bennett, V. (1981). Proteolytic domains of the epidermal growth factor receptor of human placenta. J Supramol Struct Cell Biochem, 15(1), 15–27. https://doi.org/10.1002/jsscb.1981.380150103
O’Keefe, E. J., T. Battin, and V. Bennett. “Proteolytic domains of the epidermal growth factor receptor of human placenta.J Supramol Struct Cell Biochem 15, no. 1 (1981): 15–27. https://doi.org/10.1002/jsscb.1981.380150103.
O’Keefe EJ, Battin T, Bennett V. Proteolytic domains of the epidermal growth factor receptor of human placenta. J Supramol Struct Cell Biochem. 1981;15(1):15–27.
O’Keefe, E. J., et al. “Proteolytic domains of the epidermal growth factor receptor of human placenta.J Supramol Struct Cell Biochem, vol. 15, no. 1, 1981, pp. 15–27. Pubmed, doi:10.1002/jsscb.1981.380150103.
O’Keefe EJ, Battin T, Bennett V. Proteolytic domains of the epidermal growth factor receptor of human placenta. J Supramol Struct Cell Biochem. 1981;15(1):15–27.

Published In

J Supramol Struct Cell Biochem

DOI

ISSN

0275-3723

Publication Date

1981

Volume

15

Issue

1

Start / End Page

15 / 27

Location

United States

Related Subject Headings

  • Trypsin
  • Receptors, Cell Surface
  • Pregnancy
  • Placenta
  • Peptides
  • Peptide Fragments
  • Papain
  • Microsomes
  • Kinetics
  • Intracellular Membranes