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Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype.

Publication ,  Journal Article
Schwinn, DA; Lomasney, JW; Lorenz, W; Szklut, PJ; Fremeau, RT; Yang-Feng, TL; Caron, MG; Lefkowitz, RJ; Cotecchia, S
Published in: J Biol Chem
May 15, 1990

A novel alpha 1-adrenergic receptor subtype has been cloned from a bovine brain cDNA library. The deduced amino acid sequence is that of a 466-residue polypeptide. The structure is similar to that of the other adrenergic receptors as well as the larger family of G protein-coupled receptors that have a presumed seven-membrane-spanning domain topography. The greatest sequence identity of this receptor protein is with the previously cloned hamster alpha 1B-adrenergic receptor being approximately 72% within the presumed membrane-spanning domains. Localization on different human chromosomes provides evidence that the bovine cDNA is distinct from the hamster alpha 1B-adrenergic receptor. The bovine cDNA clone expressed in COS7 cells revealed 10-fold higher affinity for the alpha 1-adrenergic antagonists WB4101 and phentolamine and the agonist oxymetazoline as compared with the alpha 1B receptor, results similar to pharmacologic binding properties described for the alpha 1A receptor. Despite these similarities in pharmacological profiles, the bovine alpha 1-adrenergic receptor is sensitive to inhibition by the alkylating agent chloroethylclonidine unlike the alpha 1A-adrenergic receptor subtype. In addition, a lack of expression in tissues where the alpha 1A subtype exists suggests that this receptor may actually represent a novel alpha 1-adrenergic receptor subtype not previously appreciated by pharmacological criteria.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 15, 1990

Volume

265

Issue

14

Start / End Page

8183 / 8189

Location

United States

Related Subject Headings

  • Transfection
  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Receptors, Adrenergic, alpha
  • Phosphorylation
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Humans
  • Glycosylation
  • Gene Expression
 

Citation

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Schwinn, D. A., Lomasney, J. W., Lorenz, W., Szklut, P. J., Fremeau, R. T., Yang-Feng, T. L., … Cotecchia, S. (1990). Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype. J Biol Chem, 265(14), 8183–8189.
Schwinn, D. A., J. W. Lomasney, W. Lorenz, P. J. Szklut, R. T. Fremeau, T. L. Yang-Feng, M. G. Caron, R. J. Lefkowitz, and S. Cotecchia. “Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype.J Biol Chem 265, no. 14 (May 15, 1990): 8183–89.
Schwinn DA, Lomasney JW, Lorenz W, Szklut PJ, Fremeau RT, Yang-Feng TL, et al. Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype. J Biol Chem. 1990 May 15;265(14):8183–9.
Schwinn, D. A., et al. “Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype.J Biol Chem, vol. 265, no. 14, May 1990, pp. 8183–89.
Schwinn DA, Lomasney JW, Lorenz W, Szklut PJ, Fremeau RT, Yang-Feng TL, Caron MG, Lefkowitz RJ, Cotecchia S. Molecular cloning and expression of the cDNA for a novel alpha 1-adrenergic receptor subtype. J Biol Chem. 1990 May 15;265(14):8183–8189.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

May 15, 1990

Volume

265

Issue

14

Start / End Page

8183 / 8189

Location

United States

Related Subject Headings

  • Transfection
  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Receptors, Adrenergic, alpha
  • Phosphorylation
  • Nucleic Acid Hybridization
  • Molecular Sequence Data
  • Humans
  • Glycosylation
  • Gene Expression