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Monensin enhances the cytotoxic effect of antitransferrin receptor immunotoxin on cultured RPE cells.

Publication ,  Journal Article
Handa, JT; Houston, LL; Jaffe, GJ
Published in: Curr Eye Res
January 1993

The effect of monensin on the cytotoxic effect of antitransferrin receptor immunotoxin (IT) was determined on cultured, human retinal pigment epithelial (hRPE) cells. Human RPE cells were treated with 0.1-10,000 ng/ml IT with and without 0.01-0.1 microM monensin, a lysosomotropic reagent that can influence IT activity. Monensin (0.01 microM) shortened the onset of cell kill with IT (10,000 ng/ml) from 48 to 24 hours (p = 0.0016). Although 0.01 microM monensin alone was not cytotoxic to hRPE cells, a single 7-day treatment with monensin caused up to a 4.1-fold increase in antiproliferative potency of IT on proliferating hRPE cells (p < or = 0.0001). Enhancement was obtained with only a 1-hour exposure to 0.1 microM monensin (p = 0.0001). In contrast, IT (0.1-10,000 ng/ml) combined with monensin (0.01 microM) had minimal effect on density-arrested cells. IT with or without monensin did not inhibit proliferation of Rhesus monkey RPE cells. Our results indicate that monensin enhances the selective cytotoxic effect of IT on proliferating hRPE cells in culture.

Duke Scholars

Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

January 1993

Volume

12

Issue

1

Start / End Page

45 / 53

Location

England

Related Subject Headings

  • Ricin
  • Recombinant Proteins
  • Receptors, Transferrin
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Monensin
  • Macaca mulatta
  • Immunotoxins
  • Humans
  • Cytotoxicity, Immunologic
 

Citation

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ICMJE
MLA
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Handa, J. T., Houston, L. L., & Jaffe, G. J. (1993). Monensin enhances the cytotoxic effect of antitransferrin receptor immunotoxin on cultured RPE cells. Curr Eye Res, 12(1), 45–53. https://doi.org/10.3109/02713689308999495
Handa, J. T., L. L. Houston, and G. J. Jaffe. “Monensin enhances the cytotoxic effect of antitransferrin receptor immunotoxin on cultured RPE cells.Curr Eye Res 12, no. 1 (January 1993): 45–53. https://doi.org/10.3109/02713689308999495.
Handa JT, Houston LL, Jaffe GJ. Monensin enhances the cytotoxic effect of antitransferrin receptor immunotoxin on cultured RPE cells. Curr Eye Res. 1993 Jan;12(1):45–53.
Handa, J. T., et al. “Monensin enhances the cytotoxic effect of antitransferrin receptor immunotoxin on cultured RPE cells.Curr Eye Res, vol. 12, no. 1, Jan. 1993, pp. 45–53. Pubmed, doi:10.3109/02713689308999495.
Handa JT, Houston LL, Jaffe GJ. Monensin enhances the cytotoxic effect of antitransferrin receptor immunotoxin on cultured RPE cells. Curr Eye Res. 1993 Jan;12(1):45–53.
Journal cover image

Published In

Curr Eye Res

DOI

ISSN

0271-3683

Publication Date

January 1993

Volume

12

Issue

1

Start / End Page

45 / 53

Location

England

Related Subject Headings

  • Ricin
  • Recombinant Proteins
  • Receptors, Transferrin
  • Pigment Epithelium of Eye
  • Ophthalmology & Optometry
  • Monensin
  • Macaca mulatta
  • Immunotoxins
  • Humans
  • Cytotoxicity, Immunologic