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Organization of the human zeta-crystallin/quinone reductase gene (CRYZ).

Publication ,  Journal Article
Gonzalez, P; Rao, PV; Zigler, JS
Published in: Genomics
May 15, 1994

zeta-Crystallin is a protein highly expressed in the lens of guinea pigs and camels, where it comprises about 10% of the total soluble protein. It has recently been characterized as a novel quinone oxidoreductase present in a variety of mammalian tissues. We report here the isolation and characterization of the human zeta-crystallin gene (CRYZ) and its processed pseudogene. The functional gene is composed of nine exons and spans about 20 kb. The 5'-flanking region of the gene is rich in G and C (58%) and lacks TATA and CAAT boxes. Previous analysis of the guinea pig gene revealed the presence of two different promoters, one responsible for the high lens-specific expression and the other for expression at the enzymatic level in numerous tissues. Comparative analysis with the guinea pig gene shows that a region of approximately 2.5 kb that includes the promoter responsible for the high expression in the lens in guinea pig is not present in the human gene.

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Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

May 15, 1994

Volume

21

Issue

2

Start / End Page

317 / 324

Location

United States

Related Subject Headings

  • TATA Box
  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Pseudogenes
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • NAD(P)H Dehydrogenase (Quinone)
  • Molecular Sequence Data
  • Liver
  • Lens, Crystalline
 

Citation

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Gonzalez, P., Rao, P. V., & Zigler, J. S. (1994). Organization of the human zeta-crystallin/quinone reductase gene (CRYZ). Genomics, 21(2), 317–324. https://doi.org/10.1006/geno.1994.1272
Gonzalez, P., P. V. Rao, and J. S. Zigler. “Organization of the human zeta-crystallin/quinone reductase gene (CRYZ).Genomics 21, no. 2 (May 15, 1994): 317–24. https://doi.org/10.1006/geno.1994.1272.
Gonzalez P, Rao PV, Zigler JS. Organization of the human zeta-crystallin/quinone reductase gene (CRYZ). Genomics. 1994 May 15;21(2):317–24.
Gonzalez, P., et al. “Organization of the human zeta-crystallin/quinone reductase gene (CRYZ).Genomics, vol. 21, no. 2, May 1994, pp. 317–24. Pubmed, doi:10.1006/geno.1994.1272.
Gonzalez P, Rao PV, Zigler JS. Organization of the human zeta-crystallin/quinone reductase gene (CRYZ). Genomics. 1994 May 15;21(2):317–324.
Journal cover image

Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

May 15, 1994

Volume

21

Issue

2

Start / End Page

317 / 324

Location

United States

Related Subject Headings

  • TATA Box
  • Sequence Homology, Nucleic Acid
  • Restriction Mapping
  • Pseudogenes
  • Promoter Regions, Genetic
  • Polymerase Chain Reaction
  • NAD(P)H Dehydrogenase (Quinone)
  • Molecular Sequence Data
  • Liver
  • Lens, Crystalline