Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing.
Publication
, Journal Article
Trammell, SA; Goldston, HM; Tran, PT; Tender, LM; Conrad, DW; Benson, DE; Hellinga, HW
Published in: Bioconjug Chem
2001
Synthesis of a novel sulfhydryl-specific, tetraammine Ru(II)polypyridyl complex, [Ru(II)(NH(3))(4)(1,10-phenanthroline-5-maleimide)](PF(6))(2), which exhibits environment-sensitive electrochemical properties is described. When conjugated to an allosteric site in a genetically engineered mutant of maltose binding protein, the formal potential of the conjugated redox probe is shifted to higher potential upon maltose binding. The magnitude of this potential shift was used to measure maltose affinity of the protein-redox conjugate complex and to monitor maltose concentration in solution. These results are presented in context of reagentless biosensing.
Duke Scholars
Published In
Bioconjug Chem
DOI
ISSN
1043-1802
Publication Date
2001
Volume
12
Issue
4
Start / End Page
643 / 647
Location
United States
Related Subject Headings
- Ruthenium
- Protein Engineering
- Protein Binding
- Phenanthrolines
- Oxidation-Reduction
- Organic Chemistry
- Maltose-Binding Proteins
- Maltose
- Maleimides
- Chemistry, Physical
Citation
APA
Chicago
ICMJE
MLA
NLM
Trammell, S. A., Goldston, H. M., Tran, P. T., Tender, L. M., Conrad, D. W., Benson, D. E., & Hellinga, H. W. (2001). Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing. Bioconjug Chem, 12(4), 643–647. https://doi.org/10.1021/bc010022q
Trammell, S. A., H. M. Goldston, P. T. Tran, L. M. Tender, D. W. Conrad, D. E. Benson, and H. W. Hellinga. “Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing.” Bioconjug Chem 12, no. 4 (2001): 643–47. https://doi.org/10.1021/bc010022q.
Trammell SA, Goldston HM, Tran PT, Tender LM, Conrad DW, Benson DE, et al. Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing. Bioconjug Chem. 2001;12(4):643–7.
Trammell, S. A., et al. “Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing.” Bioconjug Chem, vol. 12, no. 4, 2001, pp. 643–47. Pubmed, doi:10.1021/bc010022q.
Trammell SA, Goldston HM, Tran PT, Tender LM, Conrad DW, Benson DE, Hellinga HW. Synthesis and characterization of a ruthenium(II)-based redox conjugate for reagentless biosensing. Bioconjug Chem. 2001;12(4):643–647.
Published In
Bioconjug Chem
DOI
ISSN
1043-1802
Publication Date
2001
Volume
12
Issue
4
Start / End Page
643 / 647
Location
United States
Related Subject Headings
- Ruthenium
- Protein Engineering
- Protein Binding
- Phenanthrolines
- Oxidation-Reduction
- Organic Chemistry
- Maltose-Binding Proteins
- Maltose
- Maleimides
- Chemistry, Physical