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Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA.

Publication ,  Journal Article
Kanayama, S; Liddle, RA
Published in: Am J Physiol
March 1990

The gastrointestinal peptides, cholecystokinin (CCK) and somatostatin, are produced by discrete endocrine cells in the mucosa of the small intestine. Although somatostatin may inhibit CCK secretion, the mechanism by which this occurs is unknown. The present study was designed to determine the effect of somatostatin on intestinal CCK and somatostatin mRNA levels. Rats, prepared with indwelling intraduodenal and jugular cannulas, were first fed an elemental diet that did not stimulate CCK release. Next, as a means of stimulating CCK secretion, soybean trypsin inhibitor was added to the diet and perfused intraduodenally at 50 mg/h for 24 h. Trypsin inhibitor caused an 11-fold increase in plasma CCK levels and a 2.4-fold increase in duodenal CCK mRNA levels. Simultaneous intravenous infusion of somatostatin-14 (1-100 micrograms.kg-1.h-1) reduced trypsin inhibitor-stimulated CCK levels by 50% and lowered trypsin inhibitor-stimulated CCK mRNA levels by 58%. Somatostatin infusion also reduced intestinal somatostatin mRNA levels stimulated by trypsin inhibitor but did not affect basal somatostatin mRNA levels. Duodenal CCK and somatostatin peptide concentrations did not change under any of the experimental conditions. These studies demonstrate that somatostatin reduces duodenal CCK mRNA levels stimulated by diet and suggest that somatostatin itself inhibits duodenal somatostatin gene expression.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

March 1990

Volume

258

Issue

3 Pt 1

Start / End Page

G358 / G364

Location

United States

Related Subject Headings

  • Somatostatin
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Muscle, Smooth
  • Male
  • Intestinal Mucosa
  • Immunoblotting
  • Gene Expression Regulation
  • Duodenum
 

Citation

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Kanayama, S., & Liddle, R. A. (1990). Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA. Am J Physiol, 258(3 Pt 1), G358–G364. https://doi.org/10.1152/ajpgi.1990.258.3.G358
Kanayama, S., and R. A. Liddle. “Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA.Am J Physiol 258, no. 3 Pt 1 (March 1990): G358–64. https://doi.org/10.1152/ajpgi.1990.258.3.G358.
Kanayama S, Liddle RA. Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA. Am J Physiol. 1990 Mar;258(3 Pt 1):G358–64.
Kanayama, S., and R. A. Liddle. “Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA.Am J Physiol, vol. 258, no. 3 Pt 1, Mar. 1990, pp. G358–64. Pubmed, doi:10.1152/ajpgi.1990.258.3.G358.
Kanayama S, Liddle RA. Somatostatin regulates duodenal cholecystokinin and somatostatin messenger RNA. Am J Physiol. 1990 Mar;258(3 Pt 1):G358–G364.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

March 1990

Volume

258

Issue

3 Pt 1

Start / End Page

G358 / G364

Location

United States

Related Subject Headings

  • Somatostatin
  • Rats, Inbred Strains
  • Rats
  • RNA, Messenger
  • Muscle, Smooth
  • Male
  • Intestinal Mucosa
  • Immunoblotting
  • Gene Expression Regulation
  • Duodenum