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Evidence for indirect dietary regulation of cholecystokinin release in rats.

Publication ,  Journal Article
Sharara, AI; Bouras, EP; Misukonis, MA; Liddle, RA
Published in: Am J Physiol
July 1993

Food ingestion stimulates cholecystokinin (CCK) release from the proximal intestine, but the mechanisms involved are not well understood. To investigate this effect in vivo in intact rats, plasma CCK was measured after orogastric feeding of proteins, protein hydrolysates, amino acids, glucose, and starch. Intact proteins were the only nutrients to stimulate CCK release. The possibility of direct interaction between different dietary constituents and intestinal CCK-secreting endocrine cells was then examined using a perfusion system containing isolated mucosal cells from the rat duodenojejunum. The functional validity of this system was established by demonstrating that monitor peptide and bombesin both stimulated CCK release in a dose-dependent manner. The stimulatory effect of bombesin required extracellular calcium and was not inhibited by addition of tetrodotoxin. Perifusion of proteins, protein digests, and carbohydrates did not stimulate CCK release. These results indicate that proteins stimulate CCK release postprandially via an indirect mechanism, most likely related to inhibition of intraluminal trypsin. Perifusion of dispersed mucosal cells constitutes a reproducible model to investigate hormonal and peptidergic regulation of CCK release in vitro.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

July 1993

Volume

265

Issue

1 Pt 1

Start / End Page

G107 / G112

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Male
  • Intestinal Mucosa
  • Food
  • Fatty Acids
  • Digestion
  • Diet
  • Cholecystokinin
  • Caseins
 

Citation

APA
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ICMJE
MLA
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Sharara, A. I., Bouras, E. P., Misukonis, M. A., & Liddle, R. A. (1993). Evidence for indirect dietary regulation of cholecystokinin release in rats. Am J Physiol, 265(1 Pt 1), G107–G112. https://doi.org/10.1152/ajpgi.1993.265.1.G107
Sharara, A. I., E. P. Bouras, M. A. Misukonis, and R. A. Liddle. “Evidence for indirect dietary regulation of cholecystokinin release in rats.Am J Physiol 265, no. 1 Pt 1 (July 1993): G107–12. https://doi.org/10.1152/ajpgi.1993.265.1.G107.
Sharara AI, Bouras EP, Misukonis MA, Liddle RA. Evidence for indirect dietary regulation of cholecystokinin release in rats. Am J Physiol. 1993 Jul;265(1 Pt 1):G107–12.
Sharara, A. I., et al. “Evidence for indirect dietary regulation of cholecystokinin release in rats.Am J Physiol, vol. 265, no. 1 Pt 1, July 1993, pp. G107–12. Pubmed, doi:10.1152/ajpgi.1993.265.1.G107.
Sharara AI, Bouras EP, Misukonis MA, Liddle RA. Evidence for indirect dietary regulation of cholecystokinin release in rats. Am J Physiol. 1993 Jul;265(1 Pt 1):G107–G112.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

July 1993

Volume

265

Issue

1 Pt 1

Start / End Page

G107 / G112

Location

United States

Related Subject Headings

  • Rats, Sprague-Dawley
  • Rats
  • Male
  • Intestinal Mucosa
  • Food
  • Fatty Acids
  • Digestion
  • Diet
  • Cholecystokinin
  • Caseins