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Atropine enhances food-stimulated CCK secretion in the rat.

Publication ,  Journal Article
Nakano, I; Tawil, T; Spannagel, AW; Liddle, RA; Green, GM
Published in: Pancreas
September 1990

The effect of atropine on plasma cholecystokinin (CCK) and pancreatic secretion during intraintestinal infusion of a conventional defined formula liquid diet (Ensure HN, Ross Laboratories, 1.06 kcal/ml) was studied in conscious rats. Rats were prepared with cannulae draining bile and pancreatic juice, which were returned to the duodenum at all times. Pancreatic secretion was monitored during intraduodenal infusion of 0.15 M NaCl for 2 h followed by Ensure HN, both infused at 4.62 ml/h. Rats were infused i.p. with atropine (500 micrograms/kg/h) or vehicle throughout the experiment, beginning 1 h before monitoring of basal pancreatic secretion. Basal and 15 min postprandial plasma CCK concentrations were determined by bioassay. Atropine inhibited basal pancreatic protein secretion by approximately 60%. However, protein secretion during infusion of the diet was not decreased by atropine, due to a larger incremental pancreatic protein secretory response in atropine-treated rats. Plasma CCK 15 min after beginning the diet infusion was significantly increased by atropine (8.09 +/- 1.77 pM in atropine-treated rats versus 3.14 +/- 0.64 pM in controls). The results indicate that rats compensate for loss of cholinergic input to the pancreas by increasing CCK release in response to a meal. This is hypothesized to occur by virtue of reduced feedback inhibition of CCK release due to anticholinergic reduction of basal levels of intestinal protease activity.

Duke Scholars

Published In

Pancreas

DOI

ISSN

0885-3177

Publication Date

September 1990

Volume

5

Issue

5

Start / End Page

621 / 625

Location

United States

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Proteins
  • Pancreatic Juice
  • Pancreas
  • Male
  • Gastroenterology & Hepatology
  • Food
  • Cholecystokinin
  • Atropine
 

Citation

APA
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Nakano, I., Tawil, T., Spannagel, A. W., Liddle, R. A., & Green, G. M. (1990). Atropine enhances food-stimulated CCK secretion in the rat. Pancreas, 5(5), 621–625. https://doi.org/10.1097/00006676-199009000-00020
Nakano, I., T. Tawil, A. W. Spannagel, R. A. Liddle, and G. M. Green. “Atropine enhances food-stimulated CCK secretion in the rat.Pancreas 5, no. 5 (September 1990): 621–25. https://doi.org/10.1097/00006676-199009000-00020.
Nakano I, Tawil T, Spannagel AW, Liddle RA, Green GM. Atropine enhances food-stimulated CCK secretion in the rat. Pancreas. 1990 Sep;5(5):621–5.
Nakano, I., et al. “Atropine enhances food-stimulated CCK secretion in the rat.Pancreas, vol. 5, no. 5, Sept. 1990, pp. 621–25. Pubmed, doi:10.1097/00006676-199009000-00020.
Nakano I, Tawil T, Spannagel AW, Liddle RA, Green GM. Atropine enhances food-stimulated CCK secretion in the rat. Pancreas. 1990 Sep;5(5):621–625.

Published In

Pancreas

DOI

ISSN

0885-3177

Publication Date

September 1990

Volume

5

Issue

5

Start / End Page

621 / 625

Location

United States

Related Subject Headings

  • Rats, Inbred Strains
  • Rats
  • Proteins
  • Pancreatic Juice
  • Pancreas
  • Male
  • Gastroenterology & Hepatology
  • Food
  • Cholecystokinin
  • Atropine