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Regulation of cholecystokinin secretion in STC-1 cells by nitric oxide.

Publication ,  Journal Article
Mangel, AW; Scott, L; Prpic, V; Liddle, RA
Published in: Am J Physiol
October 1996

In the present study we evaluated the effects of agents anticipated to change NO levels on the secretion of cholecystokinin (CCK) from STC-1 cells. After a 15-min treatment with the nitric oxide (NO) generating agent sodium nitroprusside (SNP; 10 microM), a 24% inhibition in basal CCK release and an increase in cellular guanosine 3',5'-cyclic monophosphate (cGMP) levels were noted. By contrast, SNP (10 microM) had no effect on CCK release stimulated by L-phenylalanine (20 mM). Inhibition of NO synthase (NOS) with NG-nitro-L-arginine methyl ester (L-NAME) produced dose-dependent stimulation in CCK release. L-NAME (100-400 microM) also inhibited ATP-sensitive potassium (KATP) channels in cell-attached patches. Pretreatment of cells with disopyramide (200 microM), a KATP channel blocker, blocked L-NAME stimulation of CCK release. After inhibition of potassium channel activity by L-NAME, addition of the nonhydrolyzable cGMP analogue 8-bromo-cGMP (1-2 mM) reactivated potassium channels. NO-generating agents had no effect on channel activity in inside-out membrane patches. It is concluded that NO may serve as an important regulator of basal CCK release.

Duke Scholars

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

October 1996

Volume

271

Issue

4 Pt 1

Start / End Page

G650 / G654

Location

United States

Related Subject Headings

  • Potassium Channels
  • Potassium Channel Blockers
  • Potassium
  • Nitroprusside
  • Nitric Oxide
  • Mice
  • Disopyramide
  • Diltiazem
  • Cholecystokinin
  • Cells, Cultured
 

Citation

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Mangel, A. W., Scott, L., Prpic, V., & Liddle, R. A. (1996). Regulation of cholecystokinin secretion in STC-1 cells by nitric oxide. Am J Physiol, 271(4 Pt 1), G650–G654. https://doi.org/10.1152/ajpgi.1996.271.4.G650
Mangel, A. W., L. Scott, V. Prpic, and R. A. Liddle. “Regulation of cholecystokinin secretion in STC-1 cells by nitric oxide.Am J Physiol 271, no. 4 Pt 1 (October 1996): G650–54. https://doi.org/10.1152/ajpgi.1996.271.4.G650.
Mangel AW, Scott L, Prpic V, Liddle RA. Regulation of cholecystokinin secretion in STC-1 cells by nitric oxide. Am J Physiol. 1996 Oct;271(4 Pt 1):G650–4.
Mangel, A. W., et al. “Regulation of cholecystokinin secretion in STC-1 cells by nitric oxide.Am J Physiol, vol. 271, no. 4 Pt 1, Oct. 1996, pp. G650–54. Pubmed, doi:10.1152/ajpgi.1996.271.4.G650.
Mangel AW, Scott L, Prpic V, Liddle RA. Regulation of cholecystokinin secretion in STC-1 cells by nitric oxide. Am J Physiol. 1996 Oct;271(4 Pt 1):G650–G654.

Published In

Am J Physiol

DOI

ISSN

0002-9513

Publication Date

October 1996

Volume

271

Issue

4 Pt 1

Start / End Page

G650 / G654

Location

United States

Related Subject Headings

  • Potassium Channels
  • Potassium Channel Blockers
  • Potassium
  • Nitroprusside
  • Nitric Oxide
  • Mice
  • Disopyramide
  • Diltiazem
  • Cholecystokinin
  • Cells, Cultured