Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.
Publication
, Journal Article
Miller, SE; Levenson, RM; Aldridge, C; Hester, S; Kenan, DJ; Howell, DN
Published in: Ultrastruct Pathol
1997
A correlative microscopy method for the ultrastructural analysis of focal viral tissue infections is presented. Using a confocal scanning laser microscope, foci of infection are identified in tissue sections prior to embedment; a variety of techniques can be employed for viral detection, including staining with standard histochemical reagents and fluorescently labeled antibodies. Areas of infection identified using confocal microscopy are excised from the tissue sections, embedded, and examined by transmission electron microscopy. Applications of this technique in both diagnostic and basic research settings are described.
Duke Scholars
Published In
Ultrastruct Pathol
DOI
ISSN
0191-3123
Publication Date
1997
Volume
21
Issue
2
Start / End Page
183 / 193
Location
England
Related Subject Headings
- Virus Diseases
- Virion
- Simplexvirus
- Pathology
- Microscopy, Electron
- Microscopy, Confocal
- Mice
- Liver
- Kidney
- Humans
Citation
APA
Chicago
ICMJE
MLA
NLM
Miller, S. E., Levenson, R. M., Aldridge, C., Hester, S., Kenan, D. J., & Howell, D. N. (1997). Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis. Ultrastruct Pathol, 21(2), 183–193. https://doi.org/10.3109/01913129709021317
Miller, S. E., R. M. Levenson, C. Aldridge, S. Hester, D. J. Kenan, and D. N. Howell. “Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.” Ultrastruct Pathol 21, no. 2 (1997): 183–93. https://doi.org/10.3109/01913129709021317.
Miller SE, Levenson RM, Aldridge C, Hester S, Kenan DJ, Howell DN. Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis. Ultrastruct Pathol. 1997;21(2):183–93.
Miller, S. E., et al. “Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis.” Ultrastruct Pathol, vol. 21, no. 2, 1997, pp. 183–93. Pubmed, doi:10.3109/01913129709021317.
Miller SE, Levenson RM, Aldridge C, Hester S, Kenan DJ, Howell DN. Identification of focal viral infections by confocal microscopy for subsequent ultrastructural analysis. Ultrastruct Pathol. 1997;21(2):183–193.
Published In
Ultrastruct Pathol
DOI
ISSN
0191-3123
Publication Date
1997
Volume
21
Issue
2
Start / End Page
183 / 193
Location
England
Related Subject Headings
- Virus Diseases
- Virion
- Simplexvirus
- Pathology
- Microscopy, Electron
- Microscopy, Confocal
- Mice
- Liver
- Kidney
- Humans