Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae.
Publication
, Journal Article
Abraham, SN; Land, M; Ponniah, S; Endres, R; Hasty, DL; Babu, JP
Published in: J Bacteriol
August 1992
Glycerol was found to unravel the helical conformation of Escherichia coli type 1 fimbriae without appreciable depolymerization. The linearized fimbrial polymers have a diameter of 2 nm, react strongly with a monoclonal antibody directed at an inaccessible epitope on native fimbriae, and display greater mannose-binding activity and trypsin sensitivity than native fimbriae. Removal of glycerol by dialysis results in spontaneous reassembly of the linear polymers into structures morphologically, antigenically, and functionally indistinguishable from native fimbriae.
Duke Scholars
Altmetric Attention Stats
Dimensions Citation Stats
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
August 1992
Volume
174
Issue
15
Start / End Page
5145 / 5148
Location
United States
Related Subject Headings
- Trypsin
- Protein Conformation
- Microbiology
- Glycerol
- Fimbriae, Bacterial
- Escherichia coli
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences
- 11 Medical and Health Sciences
Citation
APA
Chicago
ICMJE
MLA
NLM
Abraham, S. N., Land, M., Ponniah, S., Endres, R., Hasty, D. L., & Babu, J. P. (1992). Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae. J Bacteriol, 174(15), 5145–5148. https://doi.org/10.1128/jb.174.15.5145-5148.1992
Abraham, S. N., M. Land, S. Ponniah, R. Endres, D. L. Hasty, and J. P. Babu. “Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae.” J Bacteriol 174, no. 15 (August 1992): 5145–48. https://doi.org/10.1128/jb.174.15.5145-5148.1992.
Abraham SN, Land M, Ponniah S, Endres R, Hasty DL, Babu JP. Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae. J Bacteriol. 1992 Aug;174(15):5145–8.
Abraham, S. N., et al. “Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae.” J Bacteriol, vol. 174, no. 15, Aug. 1992, pp. 5145–48. Pubmed, doi:10.1128/jb.174.15.5145-5148.1992.
Abraham SN, Land M, Ponniah S, Endres R, Hasty DL, Babu JP. Glycerol-induced unraveling of the tight helical conformation of Escherichia coli type 1 fimbriae. J Bacteriol. 1992 Aug;174(15):5145–5148.
Published In
J Bacteriol
DOI
ISSN
0021-9193
Publication Date
August 1992
Volume
174
Issue
15
Start / End Page
5145 / 5148
Location
United States
Related Subject Headings
- Trypsin
- Protein Conformation
- Microbiology
- Glycerol
- Fimbriae, Bacterial
- Escherichia coli
- 32 Biomedical and clinical sciences
- 31 Biological sciences
- 30 Agricultural, veterinary and food sciences
- 11 Medical and Health Sciences