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Pathogenicity of Saccharomyces cerevisiae in complement factor five-deficient mice.

Publication ,  Journal Article
Byron, JK; Clemons, KV; McCusker, JH; Davis, RW; Stevens, DA
Published in: Infect Immun
February 1995

We have previously determined the relative virulence of isolates of Saccharomyces cerevisiae on the basis of differences in proliferation and resistance to clearance in CD-1 mice. These infections were not fatal. To further characterize S. cerevisiae pathogenesis, we studied a virulent clinical isolate, YJM128, and an avirulent nonclinical isolate, Y55, in C5-deficient mice. DBA/2N mice were infected intravenously with YJM128 or Y55, and temporal burdens of yeast cells in various organs were determined. After infection with 10(7) CFU, Y55 increased by 13-fold and YJM128 increased by 20-fold in the brain from day 0 to 3. In addition, YJM128 increased by 4-fold in the kidneys, whereas Y55 decreased by 16-fold. Both isolates declined in number in other organs. In all studies, 90% of mice infected with 10(7) CFU of YJM128 died between days 2 and 7, whereas no mice infected with equivalent numbers of Y55 died. No mice died after infection with 10(6) CFU of Y55 or YJM128. The importance of C5 was confirmed by studies using B10.D2/oSnJ (C5-) mice and their congenic C5+ counterparts. Again, the C5- mice were most susceptible to infection with S. cerevisiae, with 63% infected with YJM128 dying by day 7; no C5+ mice died. No Y55-infected mice died, and mean burdens in the brain at day 14 were sevenfold lower in C5+ mice than in C5- mice. Seven of 10 other S. cerevisiae isolates were also more virulent in DBA/2N than CD-1 mice, causing > or = 40% mortality. These data indicate that C5 is a critical factor in host resistance against S. cerevisiae infections and further confirm the pathogenic potential of some isolates of S. cerevisiae.

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Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

February 1995

Volume

63

Issue

2

Start / End Page

478 / 485

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Mycoses
  • Microbiology
  • Mice, Mutant Strains
  • Mice, Inbred DBA
  • Mice
  • Male
  • Complement C5
  • Animals
  • 3207 Medical microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Byron, J. K., Clemons, K. V., McCusker, J. H., Davis, R. W., & Stevens, D. A. (1995). Pathogenicity of Saccharomyces cerevisiae in complement factor five-deficient mice. Infect Immun, 63(2), 478–485. https://doi.org/10.1128/iai.63.2.478-485.1995
Byron, J. K., K. V. Clemons, J. H. McCusker, R. W. Davis, and D. A. Stevens. “Pathogenicity of Saccharomyces cerevisiae in complement factor five-deficient mice.Infect Immun 63, no. 2 (February 1995): 478–85. https://doi.org/10.1128/iai.63.2.478-485.1995.
Byron JK, Clemons KV, McCusker JH, Davis RW, Stevens DA. Pathogenicity of Saccharomyces cerevisiae in complement factor five-deficient mice. Infect Immun. 1995 Feb;63(2):478–85.
Byron, J. K., et al. “Pathogenicity of Saccharomyces cerevisiae in complement factor five-deficient mice.Infect Immun, vol. 63, no. 2, Feb. 1995, pp. 478–85. Pubmed, doi:10.1128/iai.63.2.478-485.1995.
Byron JK, Clemons KV, McCusker JH, Davis RW, Stevens DA. Pathogenicity of Saccharomyces cerevisiae in complement factor five-deficient mice. Infect Immun. 1995 Feb;63(2):478–485.

Published In

Infect Immun

DOI

ISSN

0019-9567

Publication Date

February 1995

Volume

63

Issue

2

Start / End Page

478 / 485

Location

United States

Related Subject Headings

  • Saccharomyces cerevisiae
  • Mycoses
  • Microbiology
  • Mice, Mutant Strains
  • Mice, Inbred DBA
  • Mice
  • Male
  • Complement C5
  • Animals
  • 3207 Medical microbiology