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Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.

Publication ,  Journal Article
Goldstein, AL; McCusker, JH
Published in: Yeast
October 1999

Disruption-deletion cassettes are powerful tools used to study gene function in many organisms, including Saccharomyces cerevisiae. Perhaps the most widely useful of these are the heterologous dominant drug resistance cassettes, which use antibiotic resistance genes from bacteria and fungi as selectable markers. We have created three new dominant drug resistance cassettes by replacing the kanamycin resistance (kan(r)) open reading frame from the kanMX3 and kanMX4 disruption-deletion cassettes (Wach et al., 1994) with open reading frames conferring resistance to the antibiotics hygromycin B (hph), nourseothricin (nat) and bialaphos (pat). The new cassettes, pAG25 (natMX4), pAG29 (patMX4), pAG31 (patMX3), pAG32 (hphMX4), pAG34 (hphMX3) and pAG35 (natMX3), are cloned into pFA6, and so are in all other respects identical to pFA6-kanMX3 and pFA6-kanMX4. Most tools and techniques used with the kanMX plasmids can also be used with the hph, nat and patMX containing plasmids. These new heterologous dominant drug resistance cassettes have unique antibiotic resistance phenotypes and do not affect growth when inserted into the ho locus. These attributes make the cassettes ideally suited for creating S. cerevisiae strains with multiple mutations within a single strain.

Duke Scholars

Published In

Yeast

DOI

ISSN

0749-503X

Publication Date

October 1999

Volume

15

Issue

14

Start / End Page

1541 / 1553

Location

England

Related Subject Headings

  • Transformation, Genetic
  • Streptothricins
  • Saccharomyces cerevisiae
  • Organophosphorus Compounds
  • Mutation
  • Hygromycin B
  • Genes, Dominant
  • Gene Deletion
  • Drug Resistance, Microbial
  • 3107 Microbiology
 

Citation

APA
Chicago
ICMJE
MLA
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Goldstein, A. L., & McCusker, J. H. (1999). Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast, 15(14), 1541–1553. https://doi.org/10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K
Goldstein, A. L., and J. H. McCusker. “Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.Yeast 15, no. 14 (October 1999): 1541–53. https://doi.org/10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K.
Goldstein, A. L., and J. H. McCusker. “Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.Yeast, vol. 15, no. 14, Oct. 1999, pp. 1541–53. Pubmed, doi:10.1002/(SICI)1097-0061(199910)15:14<1541::AID-YEA476>3.0.CO;2-K.
Goldstein AL, McCusker JH. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast. 1999 Oct;15(14):1541–1553.
Journal cover image

Published In

Yeast

DOI

ISSN

0749-503X

Publication Date

October 1999

Volume

15

Issue

14

Start / End Page

1541 / 1553

Location

England

Related Subject Headings

  • Transformation, Genetic
  • Streptothricins
  • Saccharomyces cerevisiae
  • Organophosphorus Compounds
  • Mutation
  • Hygromycin B
  • Genes, Dominant
  • Gene Deletion
  • Drug Resistance, Microbial
  • 3107 Microbiology