Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR.
Publication
, Journal Article
Lashkari, DA; McCusker, JH; Davis, RW
Published in: Proc Natl Acad Sci U S A
August 19, 1997
The recent ability to sequence whole genomes allows ready access to all genetic material. The approaches outlined here allow automated analysis of sequence for the synthesis of optimal primers in an automated multiplex oligonucleotide synthesizer (AMOS). The efficiency is such that all ORFs for an organism can be amplified by PCR. The resulting amplicons can be used directly in the construction of DNA arrays or can be cloned for a large variety of functional analyses. These tools allow a replacement of single-gene analysis with a highly efficient whole-genome analysis.
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Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
August 19, 1997
Volume
94
Issue
17
Start / End Page
8945 / 8947
Location
United States
Related Subject Headings
- Sequence Analysis
- Polymerase Chain Reaction
- Humans
- Genome
- DNA Primers
- Animals
Citation
APA
Chicago
ICMJE
MLA
NLM
Lashkari, D. A., McCusker, J. H., & Davis, R. W. (1997). Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR. Proc Natl Acad Sci U S A, 94(17), 8945–8947. https://doi.org/10.1073/pnas.94.17.8945
Lashkari, D. A., J. H. McCusker, and R. W. Davis. “Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR.” Proc Natl Acad Sci U S A 94, no. 17 (August 19, 1997): 8945–47. https://doi.org/10.1073/pnas.94.17.8945.
Lashkari DA, McCusker JH, Davis RW. Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR. Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8945–7.
Lashkari, D. A., et al. “Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR.” Proc Natl Acad Sci U S A, vol. 94, no. 17, Aug. 1997, pp. 8945–47. Pubmed, doi:10.1073/pnas.94.17.8945.
Lashkari DA, McCusker JH, Davis RW. Whole genome analysis: experimental access to all genome sequenced segments through larger-scale efficient oligonucleotide synthesis and PCR. Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8945–8947.
Published In
Proc Natl Acad Sci U S A
DOI
ISSN
0027-8424
Publication Date
August 19, 1997
Volume
94
Issue
17
Start / End Page
8945 / 8947
Location
United States
Related Subject Headings
- Sequence Analysis
- Polymerase Chain Reaction
- Humans
- Genome
- DNA Primers
- Animals