Skip to main content

Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment.

Publication ,  Journal Article
Toffaletti, DL; Del Poeta, M; Rude, TH; Dietrich, F; Perfect, JR
Published in: Microbiology (Reading)
April 2003

In the study of differential gene expression of Cryptococcus neoformans, a transcript of COX1 (cytochrome oxidase c subunit 1) was identified in a serotype A strain. The transcript was upregulated at 37 degrees C compared to 30 degrees C and expressed by yeasts infecting the central nervous system. Northern analysis of COX1 from the serotype A strain revealed two polycistronic transcripts, a temperature-upregulated 2.3 kb transcript and a 1.9 kb transcript that was not affected by temperature. In contrast, COX1 in a serotype D strain showed only a 1.9 kb polycistronic transcript plus a 1.6 kb monocistronic message, and temperature had no effect on the transcripts. The sequence of COX1 revealed similar coding regions between the two strains, but the serotype D strain had five introns whereas no introns were found in the serotype A strain. The serotype D strain had reduced growth rates compared to the serotype A strain at 37 degrees C, but in an AD hybrid strain the serotype D COX1 gene could support efficient high temperature growth. These studies have revealed mitochondrial molecular differences between serotype A and D strains which show evolutionary divergence. It will be important to determine whether differences in mitochondrial structure and function can influence cryptococcosis.

Duke Scholars

Published In

Microbiology (Reading)

DOI

ISSN

1350-0872

Publication Date

April 2003

Volume

149

Issue

Pt 4

Start / End Page

1041 / 1049

Location

England

Related Subject Headings

  • Temperature
  • Serotyping
  • Sequence Analysis, DNA
  • Molecular Sequence Data
  • Mitochondria
  • Microbiology
  • Karyotyping
  • Humans
  • Gene Expression Regulation, Fungal
  • Environment
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Toffaletti, D. L., Del Poeta, M., Rude, T. H., Dietrich, F., & Perfect, J. R. (2003). Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment. Microbiology (Reading), 149(Pt 4), 1041–1049. https://doi.org/10.1099/mic.0.26021-0
Toffaletti, Dena L., Maurizio Del Poeta, Thomas H. Rude, Fred Dietrich, and John R. Perfect. “Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment.Microbiology (Reading) 149, no. Pt 4 (April 2003): 1041–49. https://doi.org/10.1099/mic.0.26021-0.
Toffaletti DL, Del Poeta M, Rude TH, Dietrich F, Perfect JR. Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment. Microbiology (Reading). 2003 Apr;149(Pt 4):1041–9.
Toffaletti, Dena L., et al. “Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment.Microbiology (Reading), vol. 149, no. Pt 4, Apr. 2003, pp. 1041–49. Pubmed, doi:10.1099/mic.0.26021-0.
Toffaletti DL, Del Poeta M, Rude TH, Dietrich F, Perfect JR. Regulation of cytochrome c oxidase subunit 1 (COX1) expression in Cryptococcus neoformans by temperature and host environment. Microbiology (Reading). 2003 Apr;149(Pt 4):1041–1049.

Published In

Microbiology (Reading)

DOI

ISSN

1350-0872

Publication Date

April 2003

Volume

149

Issue

Pt 4

Start / End Page

1041 / 1049

Location

England

Related Subject Headings

  • Temperature
  • Serotyping
  • Sequence Analysis, DNA
  • Molecular Sequence Data
  • Mitochondria
  • Microbiology
  • Karyotyping
  • Humans
  • Gene Expression Regulation, Fungal
  • Environment