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Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii.

Publication ,  Journal Article
Alberti-Segui, C; Dietrich, F; Altmann-Jöhl, R; Hoepfner, D; Philippsen, P
Published in: J Cell Sci
March 2001

We have followed the migration of GFP-labelled nuclei in multinucleate hyphae of Ashbya gossypii. For the first time we could demonstrate that the mode of long range nuclear migration consists of oscillatory movements of nuclei with, on average, higher amplitudes in the direction of the growing tip. We could also show that mitotic division proceeds at a constant rate of 0. 64 microm/minute which differs from the biphasic kinetics described for the yeast Saccharomyces cerevisiae. Furthermore we were able to identify the microtubule-based motor dynein as a key element in the control of long range nuclear migration. For other filamentous fungi it had already been demonstrated that inactivating mutations in dynein led to severe problems in nuclear migration, i.e. generation of long nuclei-free hyphal tips and clusters of nuclei throughout the hyphae. This phenotype supported the view that dynein is important for the movement of nuclei towards the tip. In A. gossypii the opposite seems to be the case. A complete deletion of the dynein heavy chain gene leads to nuclear clusters exclusively at the hyphal tips and to an essentially nucleus-free network of hyphal tubes and branches. Anucleate hyphae and branches in the vicinity of nuclear clusters show actin cables and polarized actin patches, as well as microtubules. The slow growth of this dynein null mutant could be completely reverted to wild-type-like growth in the presence of benomyl, which can be explained by the observed redistribution of nuclei in the hyphal network.

Duke Scholars

Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

March 2001

Volume

114

Issue

Pt 5

Start / End Page

975 / 986

Location

England

Related Subject Headings

  • Mutagenesis
  • Mitosis
  • Microtubules
  • Microscopy, Electron
  • Dyneins
  • Developmental Biology
  • DNA Primers
  • Cytoplasm
  • Cell Nucleus
  • Benomyl
 

Citation

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Alberti-Segui, C., Dietrich, F., Altmann-Jöhl, R., Hoepfner, D., & Philippsen, P. (2001). Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii. J Cell Sci, 114(Pt 5), 975–986. https://doi.org/10.1242/jcs.114.5.975
Alberti-Segui, C., F. Dietrich, R. Altmann-Jöhl, D. Hoepfner, and P. Philippsen. “Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii.J Cell Sci 114, no. Pt 5 (March 2001): 975–86. https://doi.org/10.1242/jcs.114.5.975.
Alberti-Segui C, Dietrich F, Altmann-Jöhl R, Hoepfner D, Philippsen P. Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii. J Cell Sci. 2001 Mar;114(Pt 5):975–86.
Alberti-Segui, C., et al. “Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii.J Cell Sci, vol. 114, no. Pt 5, Mar. 2001, pp. 975–86. Pubmed, doi:10.1242/jcs.114.5.975.
Alberti-Segui C, Dietrich F, Altmann-Jöhl R, Hoepfner D, Philippsen P. Cytoplasmic dynein is required to oppose the force that moves nuclei towards the hyphal tip in the filamentous ascomycete Ashbya gossypii. J Cell Sci. 2001 Mar;114(Pt 5):975–986.
Journal cover image

Published In

J Cell Sci

DOI

ISSN

0021-9533

Publication Date

March 2001

Volume

114

Issue

Pt 5

Start / End Page

975 / 986

Location

England

Related Subject Headings

  • Mutagenesis
  • Mitosis
  • Microtubules
  • Microscopy, Electron
  • Dyneins
  • Developmental Biology
  • DNA Primers
  • Cytoplasm
  • Cell Nucleus
  • Benomyl