Skip to main content
Journal cover image

Rat sulfite oxidase antibodies cross-react with two gene family-related proteins: albumin and vitamin D-binding protein.

Publication ,  Journal Article
Bellissimo, DB; Rajagopalan, KV
Published in: Arch Biochem Biophys
November 15, 1991

Screening lambda cDNA libraries from rat liver with antibody to native rat liver sulfite oxidase (RLSO) showed cross-reaction with two proteins that belong to the same gene family: serum albumin and vitamin D-binding protein. Antibodies raised against native RLSO or sodium dodecyl sulfate-denatured protein cross-reacted with these proteins by Western blot analysis. The relative effectiveness of RLSO antibody binding was estimated to be 1/5 for rat serum albumin and 1/10 for rat vitamin D-binding protein. This result was not caused by contaminating proteins in the RLSO used for immunization as the RLSO preparation did not react with rat serum albumin antibody. RLSO antibodies, selected for their ability to bind rat serum albumin immobilized on nitrocellulose, recognized both rat serum albumin and RLSO. RLSO antibody, with albumin-reactive antibody removed, still recognized vitamin D-binding protein, suggesting that multiple determinants specific to each protein are involved in the cross-reaction. Comparison of RLSO antibody binding to the rat and human proteins indicated that the determinants were species-specific. cDNA clones identified by screening cDNA libraries with RLSO antibody demonstrated that these determinants reside in the C-terminal domain of these proteins. These results suggest that these proteins contain some common immunological features and may be evolutionarily related.

Duke Scholars

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

November 15, 1991

Volume

291

Issue

1

Start / End Page

168 / 175

Location

United States

Related Subject Headings

  • Vitamin D-Binding Protein
  • Species Specificity
  • Rats
  • Oxidoreductases Acting on Sulfur Group Donors
  • Multigene Family
  • Humans
  • Genomic Library
  • Epitopes
  • Cross Reactions
  • Blotting, Western
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Bellissimo, D. B., & Rajagopalan, K. V. (1991). Rat sulfite oxidase antibodies cross-react with two gene family-related proteins: albumin and vitamin D-binding protein. Arch Biochem Biophys, 291(1), 168–175. https://doi.org/10.1016/0003-9861(91)90120-8
Bellissimo, D. B., and K. V. Rajagopalan. “Rat sulfite oxidase antibodies cross-react with two gene family-related proteins: albumin and vitamin D-binding protein.Arch Biochem Biophys 291, no. 1 (November 15, 1991): 168–75. https://doi.org/10.1016/0003-9861(91)90120-8.
Bellissimo DB, Rajagopalan KV. Rat sulfite oxidase antibodies cross-react with two gene family-related proteins: albumin and vitamin D-binding protein. Arch Biochem Biophys. 1991 Nov 15;291(1):168–75.
Bellissimo, D. B., and K. V. Rajagopalan. “Rat sulfite oxidase antibodies cross-react with two gene family-related proteins: albumin and vitamin D-binding protein.Arch Biochem Biophys, vol. 291, no. 1, Nov. 1991, pp. 168–75. Pubmed, doi:10.1016/0003-9861(91)90120-8.
Bellissimo DB, Rajagopalan KV. Rat sulfite oxidase antibodies cross-react with two gene family-related proteins: albumin and vitamin D-binding protein. Arch Biochem Biophys. 1991 Nov 15;291(1):168–175.
Journal cover image

Published In

Arch Biochem Biophys

DOI

ISSN

0003-9861

Publication Date

November 15, 1991

Volume

291

Issue

1

Start / End Page

168 / 175

Location

United States

Related Subject Headings

  • Vitamin D-Binding Protein
  • Species Specificity
  • Rats
  • Oxidoreductases Acting on Sulfur Group Donors
  • Multigene Family
  • Humans
  • Genomic Library
  • Epitopes
  • Cross Reactions
  • Blotting, Western