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Metal sites of copper-zinc superoxide dismutase.

Publication ,  Journal Article
Beem, KM; Richardson, DC; Rajagopalan, KV
Published in: Biochemistry
May 3, 1977

Silver-copper and silver-cobalt proteins have been prepared in which Ag+ resides in the native copper site of superoxide dismutase and either Cu2+ of Co2+ reside in the zinc site. The electron paramagnetic resonance (EPR) spectrum of the copper and the visible absorption spectrum of the cobalt greatly resemble those of either Cu4 of Cu2,Cu2,Co2 proteins, respectively, in which the copper of the native copper sites has been reduced. It was found that, unlike cyanide, azide anion would not perturb the EPR spectrum of Ag2,Cu2 protein. Since azide produces the same perturbation upon the EPR spectrum of native and Cu2 proteins, it must bind to the copper and not the zinc of superoxide dismutase. A model of the metal sites of the enzyme has been fitted to a 3-A electron-density map using an interactive molecular graphics display. The model shows that histidine-61, which appears to bind both copper and zinc, does not lie in the plane of the copper and its three other histidine ligands, but occupies a position intermediate between planar and axial. This feature probably accounts for the rhombicity of the EPR spectrum and the activity of the enzyme.

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

May 3, 1977

Volume

16

Issue

9

Start / End Page

1930 / 1936

Location

United States

Related Subject Headings

  • Zinc
  • Superoxide Dismutase
  • Silver
  • Ligands
  • Electron Spin Resonance Spectroscopy
  • Copper
  • Cobalt
  • Biochemistry & Molecular Biology
  • Azides
  • 3404 Medicinal and biomolecular chemistry
 

Citation

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Beem, K. M., Richardson, D. C., & Rajagopalan, K. V. (1977). Metal sites of copper-zinc superoxide dismutase. Biochemistry, 16(9), 1930–1936. https://doi.org/10.1021/bi00628a027
Beem, K. M., D. C. Richardson, and K. V. Rajagopalan. “Metal sites of copper-zinc superoxide dismutase.Biochemistry 16, no. 9 (May 3, 1977): 1930–36. https://doi.org/10.1021/bi00628a027.
Beem KM, Richardson DC, Rajagopalan KV. Metal sites of copper-zinc superoxide dismutase. Biochemistry. 1977 May 3;16(9):1930–6.
Beem, K. M., et al. “Metal sites of copper-zinc superoxide dismutase.Biochemistry, vol. 16, no. 9, May 1977, pp. 1930–36. Pubmed, doi:10.1021/bi00628a027.
Beem KM, Richardson DC, Rajagopalan KV. Metal sites of copper-zinc superoxide dismutase. Biochemistry. 1977 May 3;16(9):1930–1936.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

May 3, 1977

Volume

16

Issue

9

Start / End Page

1930 / 1936

Location

United States

Related Subject Headings

  • Zinc
  • Superoxide Dismutase
  • Silver
  • Ligands
  • Electron Spin Resonance Spectroscopy
  • Copper
  • Cobalt
  • Biochemistry & Molecular Biology
  • Azides
  • 3404 Medicinal and biomolecular chemistry