Skip to main content

Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides.

Publication ,  Journal Article
Hilton, JC; Rajagopalan, KV
Published in: Biochim Biophys Acta
May 23, 1996

The dsrA gene encoding the molybdoenzyme dimethyl sulfoxide reductase was isolated by screening phagemid libraries containing restriction fragments of Rhodobacter sphaeroides f. sp. denitrificans genomic DNA with a pool of degenerate oligonucleotides. The encoded 822 amino-acid protein includes a 42 amino-acid periplasmic signal sequence that is cleaved during activation of the enzyme. Both forms of the protein were heterologously expressed in inactive states in E. coli and identified by Western blot analysis.

Duke Scholars

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

May 23, 1996

Volume

1294

Issue

2

Start / End Page

111 / 114

Location

Netherlands

Related Subject Headings

  • Rhodobacter sphaeroides
  • Recombinant Proteins
  • Polymerase Chain Reaction
  • Oxidoreductases
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Iron-Sulfur Proteins
  • Genetic Vectors
  • Genes, Bacterial
  • DNA Primers
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Hilton, J. C., & Rajagopalan, K. V. (1996). Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides. Biochim Biophys Acta, 1294(2), 111–114. https://doi.org/10.1016/0167-4838(96)00015-5
Hilton, J. C., and K. V. Rajagopalan. “Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides.Biochim Biophys Acta 1294, no. 2 (May 23, 1996): 111–14. https://doi.org/10.1016/0167-4838(96)00015-5.
Hilton JC, Rajagopalan KV. Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides. Biochim Biophys Acta. 1996 May 23;1294(2):111–4.
Hilton, J. C., and K. V. Rajagopalan. “Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides.Biochim Biophys Acta, vol. 1294, no. 2, May 1996, pp. 111–14. Pubmed, doi:10.1016/0167-4838(96)00015-5.
Hilton JC, Rajagopalan KV. Molecular cloning of dimethyl sulfoxide reductase from Rhodobacter sphaeroides. Biochim Biophys Acta. 1996 May 23;1294(2):111–114.

Published In

Biochim Biophys Acta

DOI

ISSN

0006-3002

Publication Date

May 23, 1996

Volume

1294

Issue

2

Start / End Page

111 / 114

Location

Netherlands

Related Subject Headings

  • Rhodobacter sphaeroides
  • Recombinant Proteins
  • Polymerase Chain Reaction
  • Oxidoreductases
  • Mutagenesis, Site-Directed
  • Molecular Sequence Data
  • Iron-Sulfur Proteins
  • Genetic Vectors
  • Genes, Bacterial
  • DNA Primers