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Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis.

Publication ,  Journal Article
Johnson, ME; Rajagopalan, KV
Published in: J Bacteriol
January 1987

All molybdenum enzymes except nitrogenase contain a common molybdenum cofactor, whose organic moiety is a novel pterin called molybdopterin (MPT). To assist in elucidating the biosynthetic pathway of MPT, two MPT-deficient mutants of Escherichia coli K-12 were isolated. They lacked activities of the molybdenum enzymes nitrate reductase and formate dehydrogenase, did not reconstitute apo nitrate reductase from a Neurospora crassa nit-1 strain, and did not yield form A, a derivative of MPT. By P1 mapping, these two mutations mapped to chlA and chlE, loci previously postulated but never definitely shown to be involved in MPT biosynthesis. The two new mutations are in different genetic complementation groups from previously isolated chlA and chlE mutations and have been designated as chlM and chlN (closely linked to chlA and chlE, respectively). The reported presence of Mo cofactor activity in the chlA1 strain is shown to be due to in vitro synthesis of MPT through complementation between a trypsin-sensitive macromolecule from the chlA1 strain and a low-molecular-weight compound from the nit-l strain.

Duke Scholars

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1987

Volume

169

Issue

1

Start / End Page

117 / 125

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Pteridines
  • Nitrogenase
  • Nitrate Reductases
  • Nitrate Reductase
  • Neurospora crassa
  • Mutation
  • Molybdenum Cofactors
  • Molybdenum
  • Microbiology
 

Citation

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Johnson, M. E., & Rajagopalan, K. V. (1987). Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis. J Bacteriol, 169(1), 117–125. https://doi.org/10.1128/jb.169.1.117-125.1987
Johnson, M. E., and K. V. Rajagopalan. “Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis.J Bacteriol 169, no. 1 (January 1987): 117–25. https://doi.org/10.1128/jb.169.1.117-125.1987.
Johnson ME, Rajagopalan KV. Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis. J Bacteriol. 1987 Jan;169(1):117–25.
Johnson, M. E., and K. V. Rajagopalan. “Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis.J Bacteriol, vol. 169, no. 1, Jan. 1987, pp. 117–25. Pubmed, doi:10.1128/jb.169.1.117-125.1987.
Johnson ME, Rajagopalan KV. Involvement of chlA, E, M, and N loci in Escherichia coli molybdopterin biosynthesis. J Bacteriol. 1987 Jan;169(1):117–125.

Published In

J Bacteriol

DOI

ISSN

0021-9193

Publication Date

January 1987

Volume

169

Issue

1

Start / End Page

117 / 125

Location

United States

Related Subject Headings

  • Spectrometry, Fluorescence
  • Pteridines
  • Nitrogenase
  • Nitrate Reductases
  • Nitrate Reductase
  • Neurospora crassa
  • Mutation
  • Molybdenum Cofactors
  • Molybdenum
  • Microbiology