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Molecular cloning of rat liver sulfite oxidase. Expression of a eukaryotic Mo-pterin-containing enzyme in Escherichia coli.

Publication ,  Journal Article
Garrett, RM; Rajagopalan, KV
Published in: J Biol Chem
January 7, 1994

The cDNA encoding sulfite oxidase has been cloned from a rat liver cDNA library. The gene contains a single open reading frame of 1464 nucleotides encoding a protein of 488 amino acids. The deduced amino acid sequence contains a 22-residue amino-terminal presequence that may serve as a mitochondrial targeting signal. The amino acid sequence deduced from the cDNA shows significant similarity to those of sulfite oxidase from chicken liver and nitrate reductases from algal, fungal, and plant sources. Two cysteine residues are conserved in all of these proteins, and it is proposed that one or both of these cysteines serve as ligands to molybdenum. The gene has been expressed in Escherichia coli to a level equivalent to that observed in rat liver. The recombinant enzyme has been found to contain the molybdopterin form of the molybdenum cofactor and is active as determined by the sulfite dependent reduction of cytochrome c.

Duke Scholars

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

January 7, 1994

Volume

269

Issue

1

Start / End Page

272 / 276

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Rats
  • Pteridines
  • Oxidoreductases Acting on Sulfur Group Donors
  • Molybdenum Cofactors
  • Molecular Sequence Data
  • Metalloproteins
  • Liver
  • Escherichia coli
  • DNA, Complementary
 

Citation

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Garrett, R. M., and K. V. Rajagopalan. “Molecular cloning of rat liver sulfite oxidase. Expression of a eukaryotic Mo-pterin-containing enzyme in Escherichia coli.J Biol Chem 269, no. 1 (January 7, 1994): 272–76.
Garrett, R. M., and K. V. Rajagopalan. “Molecular cloning of rat liver sulfite oxidase. Expression of a eukaryotic Mo-pterin-containing enzyme in Escherichia coli.J Biol Chem, vol. 269, no. 1, Jan. 1994, pp. 272–76.

Published In

J Biol Chem

ISSN

0021-9258

Publication Date

January 7, 1994

Volume

269

Issue

1

Start / End Page

272 / 276

Location

United States

Related Subject Headings

  • Sequence Homology, Amino Acid
  • Rats
  • Pteridines
  • Oxidoreductases Acting on Sulfur Group Donors
  • Molybdenum Cofactors
  • Molecular Sequence Data
  • Metalloproteins
  • Liver
  • Escherichia coli
  • DNA, Complementary