Skip to main content
Journal cover image

Cosmids map two incontinentia pigmenti type 1 (IP1) translocation breakpoints to a 180-kb region within a 1.2-Mb YAC contig.

Publication ,  Journal Article
Gorski, JL; Bialecki, MD; McDonald, MT; Massa, HF; Trask, BJ; Burright, EN
Published in: Genomics
July 15, 1996

Incontinentia pigmenti (IP) is an X-linked dominant disorder of neuroectodermal development. Based on the observation of six unrelated females with clinical features of nonfamilial IP with constitutional de novo reciprocal X;autosome translocations, a putative incontinentia pigmenti type 1 locus (IP1; MIM No. 308300) was localized to region Xp11.21. Using available regional DNA markers, we constructed a yeast artificial chromosome (YAC) contig that contained 1.2 Mb of distal Xp11.21 and spanned two IP1 X-chromosomal breakpoints. This contig was used to generate a detailed molecular map of the region and identify three regional CpG islands. YAC-derived cosmids were used to clone and map the IP1 breakpoints to a 180-kb interval that was flanked by DNA markers DXS705 and DXS741. The physical map and genomic clones should facilitate the isolation and characterization of transcripts associated with the IP1 translocation breakpoints.

Duke Scholars

Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

July 15, 1996

Volume

35

Issue

2

Start / End Page

338 / 345

Location

United States

Related Subject Headings

  • X Chromosome
  • Translocation, Genetic
  • Molecular Sequence Data
  • Incontinentia Pigmenti
  • In Situ Hybridization, Fluorescence
  • Humans
  • Genetics & Heredity
  • Genetic Markers
  • Female
  • DNA Primers
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Gorski, J. L., Bialecki, M. D., McDonald, M. T., Massa, H. F., Trask, B. J., & Burright, E. N. (1996). Cosmids map two incontinentia pigmenti type 1 (IP1) translocation breakpoints to a 180-kb region within a 1.2-Mb YAC contig. Genomics, 35(2), 338–345. https://doi.org/10.1006/geno.1996.0365
Gorski, J. L., M. D. Bialecki, M. T. McDonald, H. F. Massa, B. J. Trask, and E. N. Burright. “Cosmids map two incontinentia pigmenti type 1 (IP1) translocation breakpoints to a 180-kb region within a 1.2-Mb YAC contig.Genomics 35, no. 2 (July 15, 1996): 338–45. https://doi.org/10.1006/geno.1996.0365.
Gorski JL, Bialecki MD, McDonald MT, Massa HF, Trask BJ, Burright EN. Cosmids map two incontinentia pigmenti type 1 (IP1) translocation breakpoints to a 180-kb region within a 1.2-Mb YAC contig. Genomics. 1996 Jul 15;35(2):338–45.
Gorski, J. L., et al. “Cosmids map two incontinentia pigmenti type 1 (IP1) translocation breakpoints to a 180-kb region within a 1.2-Mb YAC contig.Genomics, vol. 35, no. 2, July 1996, pp. 338–45. Pubmed, doi:10.1006/geno.1996.0365.
Gorski JL, Bialecki MD, McDonald MT, Massa HF, Trask BJ, Burright EN. Cosmids map two incontinentia pigmenti type 1 (IP1) translocation breakpoints to a 180-kb region within a 1.2-Mb YAC contig. Genomics. 1996 Jul 15;35(2):338–345.
Journal cover image

Published In

Genomics

DOI

ISSN

0888-7543

Publication Date

July 15, 1996

Volume

35

Issue

2

Start / End Page

338 / 345

Location

United States

Related Subject Headings

  • X Chromosome
  • Translocation, Genetic
  • Molecular Sequence Data
  • Incontinentia Pigmenti
  • In Situ Hybridization, Fluorescence
  • Humans
  • Genetics & Heredity
  • Genetic Markers
  • Female
  • DNA Primers