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The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits.

Publication ,  Journal Article
Harashima, T; Heitman, J
Published in: Mol Cell
July 2002

G protein coupled receptors (GPCR) sense diverse ligands and signal via heterotrimeric G proteins. The Saccharomyces cerevisiae GPCR Gpr1 senses glucose and controls filamentous growth via an unusual Galpha protein, Gpa2, which lacks any known Gbetagamma subunits. Our genetic and biochemical studies identify Gpa2 interaction partners (Gpb1/2, Gpg1) and provide evidence that these proteins function as G protein subunit mimics and signaling effectors. Gpb1 and Gpb2 lack the seven WD-40 repeats found in Gbeta subunits and instead contain seven kelch repeats implicated in protein-protein interactions. Gbeta subunits and the kelch repeat protein galactose oxidase fold into strikingly similar seven-bladed beta propellers. Our studies demonstrate that Gpa2 signals in conjunction with Gbeta structural mimics and that homologous G protein subunits or effectors may be conserved in multicellular eukaryotes.

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Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

July 2002

Volume

10

Issue

1

Start / End Page

163 / 173

Location

United States

Related Subject Headings

  • Time Factors
  • Schizosaccharomyces pombe Proteins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repetitive Sequences, Amino Acid
  • Protein Subunits
  • Protein Conformation
  • Protein Binding
  • Molecular Sequence Data
  • Models, Molecular
 

Citation

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Harashima, T., & Heitman, J. (2002). The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits. Mol Cell, 10(1), 163–173. https://doi.org/10.1016/s1097-2765(02)00569-5
Harashima, Toshiaki, and Joseph Heitman. “The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits.Mol Cell 10, no. 1 (July 2002): 163–73. https://doi.org/10.1016/s1097-2765(02)00569-5.
Harashima, Toshiaki, and Joseph Heitman. “The Galpha protein Gpa2 controls yeast differentiation by interacting with kelch repeat proteins that mimic Gbeta subunits.Mol Cell, vol. 10, no. 1, July 2002, pp. 163–73. Pubmed, doi:10.1016/s1097-2765(02)00569-5.
Journal cover image

Published In

Mol Cell

DOI

ISSN

1097-2765

Publication Date

July 2002

Volume

10

Issue

1

Start / End Page

163 / 173

Location

United States

Related Subject Headings

  • Time Factors
  • Schizosaccharomyces pombe Proteins
  • Saccharomyces cerevisiae Proteins
  • Saccharomyces cerevisiae
  • Repetitive Sequences, Amino Acid
  • Protein Subunits
  • Protein Conformation
  • Protein Binding
  • Molecular Sequence Data
  • Models, Molecular