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An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate.

Publication ,  Journal Article
Melan, MA; Dong, X; Endara, ME; Davis, KR; Ausubel, FM; Peterman, TK
Published in: Plant physiology
February 1993

We isolated and characterized a 2.8-kb, full-length, Arabidopsis thaliana cDNA clone encoding a lipoxygenase. DNA sequence analysis showed that the deduced amino acid sequence of the Arabidopsis protein is 72 to 78% similar to that of legume seed lipoxygenases. DNA blot analysis indicated that Arabidopsis contains a single gene, LOX1, with appreciable homology to the cDNA clone. RNA blot analysis showed that the LOX1 gene is expressed in Arabidopsis leaves, roots, inflorescences, and young seedlings. LOX1 expression levels were highest in roots and young seedlings. In mature plants, LOX1 mRNA levels increased upon treatment with the stress-related hormones abscisic acid and methyl jasmonate and remained high for at least 96 h. Expression of the LOX1 gene was examined following infiltration of leaves with virulent (Psm ES4326) and avirulent (Pst MM1065) strains of Pseudomonas syringae. LOX1 mRNA levels were induced approximately 6-fold by both virulent and avirulent strains; however, the response to avirulent strains was much more rapid. Infiltration of leaves with Pst MM1065 resulted in maximal induction within 12 h, whereas maximal induction by Psm ES4326 did not occur until 48 h. When a cloned avr gene, avrRpt2, was transferred to Psm ES4326, LOX1 mRNA accumulated in a pattern similar to that observed for the avirulent strain Pst MM1065.

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Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

February 1993

Volume

101

Issue

2

Start / End Page

441 / 450

Related Subject Headings

  • Virulence
  • Tissue Distribution
  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • RNA
  • Pseudomonas
  • Plant Diseases
  • Plant Biology & Botany
  • Oxylipins
  • Molecular Sequence Data
 

Citation

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Melan, M. A., Dong, X., Endara, M. E., Davis, K. R., Ausubel, F. M., & Peterman, T. K. (1993). An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant Physiology, 101(2), 441–450. https://doi.org/10.1104/pp.101.2.441
Melan, M. A., X. Dong, M. E. Endara, K. R. Davis, F. M. Ausubel, and T. K. Peterman. “An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate.Plant Physiology 101, no. 2 (February 1993): 441–50. https://doi.org/10.1104/pp.101.2.441.
Melan MA, Dong X, Endara ME, Davis KR, Ausubel FM, Peterman TK. An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant physiology. 1993 Feb;101(2):441–50.
Melan, M. A., et al. “An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate.Plant Physiology, vol. 101, no. 2, Feb. 1993, pp. 441–50. Epmc, doi:10.1104/pp.101.2.441.
Melan MA, Dong X, Endara ME, Davis KR, Ausubel FM, Peterman TK. An Arabidopsis thaliana lipoxygenase gene can be induced by pathogens, abscisic acid, and methyl jasmonate. Plant physiology. 1993 Feb;101(2):441–450.

Published In

Plant physiology

DOI

EISSN

1532-2548

ISSN

0032-0889

Publication Date

February 1993

Volume

101

Issue

2

Start / End Page

441 / 450

Related Subject Headings

  • Virulence
  • Tissue Distribution
  • Sequence Homology, Amino Acid
  • Sequence Analysis, DNA
  • RNA
  • Pseudomonas
  • Plant Diseases
  • Plant Biology & Botany
  • Oxylipins
  • Molecular Sequence Data