Revision of standards for adjusting the cation content of Mueller-Hinton broth for testing susceptibility of Pseudomonas aeruginosa to aminoglycosides.
Publication
, Journal Article
Barry, AL; Reller, LB; Miller, GH; Washington, JA; Schoenknect, FD; Peterson, LR; Hare, RS; Knapp, C
Published in: J Clin Microbiol
March 1992
A multilaboratory study was undertaken to reassess the amount of calcium and magnesium that should be added to Mueller-Hinton broth when testing Pseudomonas aeruginosa against amikacin, gentamicin, isepamicin, netilmicin, and tobramycin. To achieve parity with agar dilution tests, cation-adjusted broth should contain 20 to 25 mg of calcium and 10 to 12.5 mg of magnesium per liter rather than the 50- and 25-mg/liter supplements recommended previously. For quality control of tests with contemporary media, MIC control limits should be adjusted by lowering the current MIC limits by at least 1 doubling-dilution interval.
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Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
March 1992
Volume
30
Issue
3
Start / End Page
585 / 589
Location
United States
Related Subject Headings
- Pseudomonas aeruginosa
- Microbiology
- Microbial Sensitivity Tests
- Magnesium
- Humans
- Culture Media
- Cations
- Anti-Bacterial Agents
- Aminoglycosides
- 3207 Medical microbiology
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Chicago
ICMJE
MLA
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Barry, A. L., Reller, L. B., Miller, G. H., Washington, J. A., Schoenknect, F. D., Peterson, L. R., … Knapp, C. (1992). Revision of standards for adjusting the cation content of Mueller-Hinton broth for testing susceptibility of Pseudomonas aeruginosa to aminoglycosides. J Clin Microbiol, 30(3), 585–589. https://doi.org/10.1128/jcm.30.3.585-589.1992
Barry, A. L., L. B. Reller, G. H. Miller, J. A. Washington, F. D. Schoenknect, L. R. Peterson, R. S. Hare, and C. Knapp. “Revision of standards for adjusting the cation content of Mueller-Hinton broth for testing susceptibility of Pseudomonas aeruginosa to aminoglycosides.” J Clin Microbiol 30, no. 3 (March 1992): 585–89. https://doi.org/10.1128/jcm.30.3.585-589.1992.
Barry AL, Reller LB, Miller GH, Washington JA, Schoenknect FD, Peterson LR, et al. Revision of standards for adjusting the cation content of Mueller-Hinton broth for testing susceptibility of Pseudomonas aeruginosa to aminoglycosides. J Clin Microbiol. 1992 Mar;30(3):585–9.
Barry, A. L., et al. “Revision of standards for adjusting the cation content of Mueller-Hinton broth for testing susceptibility of Pseudomonas aeruginosa to aminoglycosides.” J Clin Microbiol, vol. 30, no. 3, Mar. 1992, pp. 585–89. Pubmed, doi:10.1128/jcm.30.3.585-589.1992.
Barry AL, Reller LB, Miller GH, Washington JA, Schoenknect FD, Peterson LR, Hare RS, Knapp C. Revision of standards for adjusting the cation content of Mueller-Hinton broth for testing susceptibility of Pseudomonas aeruginosa to aminoglycosides. J Clin Microbiol. 1992 Mar;30(3):585–589.
Published In
J Clin Microbiol
DOI
ISSN
0095-1137
Publication Date
March 1992
Volume
30
Issue
3
Start / End Page
585 / 589
Location
United States
Related Subject Headings
- Pseudomonas aeruginosa
- Microbiology
- Microbial Sensitivity Tests
- Magnesium
- Humans
- Culture Media
- Cations
- Anti-Bacterial Agents
- Aminoglycosides
- 3207 Medical microbiology