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Use of a sodium polyanetholesulfonate disk for the identification of Gardnerella vaginalis.

Publication ,  Journal Article
Reimer, LG; Reller, LB
Published in: J Clin Microbiol
February 1985

Several methods have been previously suggested for the presumptive identification of Gardnerella vaginalis in clinical laboratories, but none is entirely satisfactory. We previously found that sodium polyanetholesulfonate (SPS) inhibits G. vaginalis in blood culture media. We compared susceptibility to an SPS-containing paper disk with beta-hemolysis on human blood agar, hippurate hydrolysis, and inhibition by alpha-hemolytic streptococci for identification of 62 previously confirmed G. vaginalis strains. All strains were positive by SPS disk and alpha-hemolytic streptococcus inhibition, 78% were positive by beta-hemolysis, and 81% were positive by hippurate hydrolysis. Although positive reactions occurred with SPS disk and alpha-hemolytic streptococcus tests for 5 and 9 of 84 other bacteria tested, respectively, none of these bacteria were positive for both tests. We conclude that a combination of SPS disk susceptibility and alpha-hemolytic streptococcus inhibition provides excellent identification of G. vaginalis when performed by the methods suggested.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

February 1985

Volume

21

Issue

2

Start / End Page

146 / 149

Location

United States

Related Subject Headings

  • Polyanetholesulfonate
  • Microbiology
  • Humans
  • Hemolysis
  • Haemophilus
  • Gardnerella vaginalis
  • Culture Media
  • Benzenesulfonates
  • Bacteriological Techniques
  • 3207 Medical microbiology
 

Citation

APA
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ICMJE
MLA
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Reimer, L. G., & Reller, L. B. (1985). Use of a sodium polyanetholesulfonate disk for the identification of Gardnerella vaginalis. J Clin Microbiol, 21(2), 146–149. https://doi.org/10.1128/jcm.21.2.146-149.1985
Reimer, L. G., and L. B. Reller. “Use of a sodium polyanetholesulfonate disk for the identification of Gardnerella vaginalis.J Clin Microbiol 21, no. 2 (February 1985): 146–49. https://doi.org/10.1128/jcm.21.2.146-149.1985.
Reimer LG, Reller LB. Use of a sodium polyanetholesulfonate disk for the identification of Gardnerella vaginalis. J Clin Microbiol. 1985 Feb;21(2):146–9.
Reimer, L. G., and L. B. Reller. “Use of a sodium polyanetholesulfonate disk for the identification of Gardnerella vaginalis.J Clin Microbiol, vol. 21, no. 2, Feb. 1985, pp. 146–49. Pubmed, doi:10.1128/jcm.21.2.146-149.1985.
Reimer LG, Reller LB. Use of a sodium polyanetholesulfonate disk for the identification of Gardnerella vaginalis. J Clin Microbiol. 1985 Feb;21(2):146–149.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

February 1985

Volume

21

Issue

2

Start / End Page

146 / 149

Location

United States

Related Subject Headings

  • Polyanetholesulfonate
  • Microbiology
  • Humans
  • Hemolysis
  • Haemophilus
  • Gardnerella vaginalis
  • Culture Media
  • Benzenesulfonates
  • Bacteriological Techniques
  • 3207 Medical microbiology