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Controlled evaluation of blood culture medium containing gelatin and V-factor-analog for detection of septicemia in children.

Publication ,  Journal Article
Stratton, CW; Weinstein, MP; Mirrett, S; Paisley, J; Lauer, BA; Reller, LB
Published in: J Clin Microbiol
April 1988

Both Neisseria meningitidis and Haemophilus influenzae are important isolates recovered in blood cultures from septicemic children. Sodium polyanetholsulfonate is present in most blood culture media and can inhibit the growth of certain bacteria, including N. meningitidis. The addition of gelatin to blood culture media neutralizes this inhibition. The growth of H. influenzae is enhanced by specific growth factors such as hemin and NAD. The addition of gelatin and V-factor-analog (a proprietary supplement for enhancing the growth of H. influenzae) might have a positive effect on the yield and on the speed of detection of septicemia in children. To evaluate this possibility, we did 4,565 paired comparisons of blood cultured in BACTEC 6B (aerobic) medium with and without the addition of both 1.2% gelatin and V-factor-analog. More aerobic and facultative bacteria grew in the 6B than in the 6B-gelatin-V-factor-analog medium (P less than 0.01). Only seven isolates of Neisseria spp. were recovered during this study period, with the 6B medium performing as well as the supplemented medium. When microorganisms grew in both bottles, they did so at the same time except for H. influenzae and Candida albicans. H. influenzae was recovered earlier from the 6B-gelatin-V-factor-analog bottle (P less than 0.01), with a mean time to detection of 8.5 h compared with 15.9 h for the 6B bottle. C. albicans was recovered earlier from the 6B bottle (P less than 0.02), with a mean time to detection of 34.9 h compared with 71.6 h for the 6B-gelatin-V-factor-analog bottle. We conclude that the 6B medium in its present formulation is superior to bB supplemented with gelatin and V-factor-analog.

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Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1988

Volume

26

Issue

4

Start / End Page

747 / 749

Location

United States

Related Subject Headings

  • Sepsis
  • Mycoses
  • Microbiology
  • Infant, Newborn
  • Infant
  • Humans
  • Growth Substances
  • Gelatin
  • Fungi
  • Culture Media
 

Citation

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MLA
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Stratton, C. W., Weinstein, M. P., Mirrett, S., Paisley, J., Lauer, B. A., & Reller, L. B. (1988). Controlled evaluation of blood culture medium containing gelatin and V-factor-analog for detection of septicemia in children. J Clin Microbiol, 26(4), 747–749. https://doi.org/10.1128/jcm.26.4.747-749.1988
Stratton, C. W., M. P. Weinstein, S. Mirrett, J. Paisley, B. A. Lauer, and L. B. Reller. “Controlled evaluation of blood culture medium containing gelatin and V-factor-analog for detection of septicemia in children.J Clin Microbiol 26, no. 4 (April 1988): 747–49. https://doi.org/10.1128/jcm.26.4.747-749.1988.
Stratton CW, Weinstein MP, Mirrett S, Paisley J, Lauer BA, Reller LB. Controlled evaluation of blood culture medium containing gelatin and V-factor-analog for detection of septicemia in children. J Clin Microbiol. 1988 Apr;26(4):747–9.
Stratton, C. W., et al. “Controlled evaluation of blood culture medium containing gelatin and V-factor-analog for detection of septicemia in children.J Clin Microbiol, vol. 26, no. 4, Apr. 1988, pp. 747–49. Pubmed, doi:10.1128/jcm.26.4.747-749.1988.
Stratton CW, Weinstein MP, Mirrett S, Paisley J, Lauer BA, Reller LB. Controlled evaluation of blood culture medium containing gelatin and V-factor-analog for detection of septicemia in children. J Clin Microbiol. 1988 Apr;26(4):747–749.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

April 1988

Volume

26

Issue

4

Start / End Page

747 / 749

Location

United States

Related Subject Headings

  • Sepsis
  • Mycoses
  • Microbiology
  • Infant, Newborn
  • Infant
  • Humans
  • Growth Substances
  • Gelatin
  • Fungi
  • Culture Media