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Controlled evaluation of the effect of atmosphere of incubation on detection of bacteremia and fungemia in supplemented peptone broth.

Publication ,  Journal Article
Tenney, JH; Reller, LB; Mirrett, S; Weinstein, MP; Wang, WL
Published in: J Clin Microbiol
September 1982

To evaluate the role of atmosphere of incubation in the detection of clinically important bacteremia and fungemia in adults, we compared the yield of microorganisms from 10,541 paired 5-ml samples of blood incubated aerobically and anaerobically. The medium, supplemented peptone broth (SPB) with 0.03% sodium polyanetholesulfonate, and the ratio of blood to broth (1:10) were the same for all cultures. Only cultures with adequate blood samples (greater than or equal to 80% of stated volume) were compared statistically. More fungi (P less than 10(-7) ) grew in continuously vented bottles of SPB. Aerobic incubation also favored (P less than 0.01) isolation of Neisseria gonorrhoeae and Eubacterium; more than 80% of these bacterial organisms were detected only in vented bottles. Anaerobic incubation (plugged venting units) did not significantly favor the isolation of any genus of microorganisms, although an estimated 11% more Bacteroidaceae grew in the unvented bottle of SPB. By comparison of our data with published results for other media, we conclude that the need for both aerobic and anaerobic incubation of blood cultures is dependent upon the medium used and the microorganisms likely to be encountered. Vented incubation of blood cultured in SPB is crucial for detection of fungi and some bacteria. Routine use of an unvented bottle of SPB may not be worthwhile for patients in whom Bacteroidaceae cause bacteremia infrequently. However, when Bacteroidaceae are suspected as the cause of sepsis, use of an unvented bottle of SPB is prudent.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

September 1982

Volume

16

Issue

3

Start / End Page

437 / 442

Location

United States

Related Subject Headings

  • Sepsis
  • Peptones
  • Mycoses
  • Microbiology
  • Microbiological Techniques
  • Fungi
  • Eubacterium
  • Culture Media
  • Bacteroidaceae
  • Bacteria
 

Citation

APA
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ICMJE
MLA
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Tenney, J. H., Reller, L. B., Mirrett, S., Weinstein, M. P., & Wang, W. L. (1982). Controlled evaluation of the effect of atmosphere of incubation on detection of bacteremia and fungemia in supplemented peptone broth. J Clin Microbiol, 16(3), 437–442. https://doi.org/10.1128/jcm.16.3.437-442.1982
Tenney, J. H., L. B. Reller, S. Mirrett, M. P. Weinstein, and W. L. Wang. “Controlled evaluation of the effect of atmosphere of incubation on detection of bacteremia and fungemia in supplemented peptone broth.J Clin Microbiol 16, no. 3 (September 1982): 437–42. https://doi.org/10.1128/jcm.16.3.437-442.1982.
Tenney JH, Reller LB, Mirrett S, Weinstein MP, Wang WL. Controlled evaluation of the effect of atmosphere of incubation on detection of bacteremia and fungemia in supplemented peptone broth. J Clin Microbiol. 1982 Sep;16(3):437–42.
Tenney, J. H., et al. “Controlled evaluation of the effect of atmosphere of incubation on detection of bacteremia and fungemia in supplemented peptone broth.J Clin Microbiol, vol. 16, no. 3, Sept. 1982, pp. 437–42. Pubmed, doi:10.1128/jcm.16.3.437-442.1982.
Tenney JH, Reller LB, Mirrett S, Weinstein MP, Wang WL. Controlled evaluation of the effect of atmosphere of incubation on detection of bacteremia and fungemia in supplemented peptone broth. J Clin Microbiol. 1982 Sep;16(3):437–442.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

September 1982

Volume

16

Issue

3

Start / End Page

437 / 442

Location

United States

Related Subject Headings

  • Sepsis
  • Peptones
  • Mycoses
  • Microbiology
  • Microbiological Techniques
  • Fungi
  • Eubacterium
  • Culture Media
  • Bacteroidaceae
  • Bacteria