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Evaluation of transport media for Campylobacter jejuni in human fecal specimens.

Publication ,  Journal Article
Wang, WL; Reller, LB; Smallwood, B; Luechtefeld, NW; Blaser, MJ
Published in: J Clin Microbiol
October 1983

It is not always possible to culture feces immediately, and appropriate methods for transport of human specimens, unlike those from animals, have not been fully evaluated. Therefore, we took serial subcultures in two phases from six transport media inoculated with human diarrheal stools known to be positive for Campylobacter jejuni. In phase 1, Cary-Blair medium and buffered glycerol saline did not preserve C. jejuni as well as did alkaline peptone-water (APW), modified Cary-Blair medium, thioglycolate broth (Thio), and Campy-Thio. The four best media (APW, Cary-Blair medium, Thio, and Campy-Thio) preserved 20 fecal samples with C. jejuni better at 4 degrees C (90% survival for 5 to 8 days) than at 25 degrees C (90% survival for 1.7 to 2 days). In phase 2, APW and Thio, along with four modifications of the best media in phase 1, were tested with 23 positive strains. The ranges of survival times with modified media at 25 degrees C were 1.3 to 2.2 days (90%) and 4.7 to 6.8 days (50%). APW with reducing agents preserved C. jejuni better than did APW alone, Thio plus ox bile, or Campy-Thio plus ox bile (P less than 0.05). Thio at pH 8.5 was better at preserving C. jejuni than was APW or Thio plus ox bile (P less than 0.05). If human fecal specimens cannot be refrigerated during transport or storage, we recommend the use of Thio at pH 8.5 or APW with reducing agents for preservation of C. jejuni at 25 degrees C.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

October 1983

Volume

18

Issue

4

Start / End Page

803 / 807

Location

United States

Related Subject Headings

  • Time Factors
  • Temperature
  • Specimen Handling
  • Microbiology
  • Humans
  • Feces
  • Culture Media
  • Campylobacter fetus
  • Bacteriological Techniques
  • 3207 Medical microbiology
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Wang, W. L., Reller, L. B., Smallwood, B., Luechtefeld, N. W., & Blaser, M. J. (1983). Evaluation of transport media for Campylobacter jejuni in human fecal specimens. J Clin Microbiol, 18(4), 803–807. https://doi.org/10.1128/jcm.18.4.803-807.1983
Wang, W. L., L. B. Reller, B. Smallwood, N. W. Luechtefeld, and M. J. Blaser. “Evaluation of transport media for Campylobacter jejuni in human fecal specimens.J Clin Microbiol 18, no. 4 (October 1983): 803–7. https://doi.org/10.1128/jcm.18.4.803-807.1983.
Wang WL, Reller LB, Smallwood B, Luechtefeld NW, Blaser MJ. Evaluation of transport media for Campylobacter jejuni in human fecal specimens. J Clin Microbiol. 1983 Oct;18(4):803–7.
Wang, W. L., et al. “Evaluation of transport media for Campylobacter jejuni in human fecal specimens.J Clin Microbiol, vol. 18, no. 4, Oct. 1983, pp. 803–07. Pubmed, doi:10.1128/jcm.18.4.803-807.1983.
Wang WL, Reller LB, Smallwood B, Luechtefeld NW, Blaser MJ. Evaluation of transport media for Campylobacter jejuni in human fecal specimens. J Clin Microbiol. 1983 Oct;18(4):803–807.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

October 1983

Volume

18

Issue

4

Start / End Page

803 / 807

Location

United States

Related Subject Headings

  • Time Factors
  • Temperature
  • Specimen Handling
  • Microbiology
  • Humans
  • Feces
  • Culture Media
  • Campylobacter fetus
  • Bacteriological Techniques
  • 3207 Medical microbiology