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Utility of paired BACTEC MYCO/F LYTIC blood culture vials for detection of bacteremia, mycobacteremia, and fungemia.

Publication ,  Journal Article
Archibald, LK; Dobbie, H; Kazembe, P; Nwanyanwu, O; McKnight, C; Byrne, T; Addison, RM; Bell, M; Reller, LB; Jarvis, WR
Published in: J Clin Microbiol
May 2001

In previous bloodstream infection studies in Malawi, we inoculated blood from a single venesection into a single BACTEC MYCO/F LYTIC (MFL) vial. Inoculation of one vial, however, would be expected to reduce the sensitivity of bloodstream pathogen detection with MFL vials. To ascertain the degree of this loss of sensitivity, blood was drawn from each of 228 febrile, adult inpatients in Malawi and 5 ml of each blood sample was inoculated into each of two MFL vials. Of 228 paired vials, 51 (22%) were both positive, 172 (75%) were both negative, and 5 (3%) had discordant results. Bloodstream infection would have been detected in 11 (92%) of 12 patients with mycobacteremia and 38 (92%) of 41 patients with bacteremia had only one MFL vial been inoculated. Our study shows that a second MFL vial does not significantly increase diagnostic sensitivity.

Duke Scholars

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

May 2001

Volume

39

Issue

5

Start / End Page

1960 / 1962

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Reagent Kits, Diagnostic
  • Mycobacterium Infections
  • Mycobacterium
  • Microbiology
  • Humans
  • Fungemia
  • Culture Media
  • Blood
  • Bacteremia
 

Citation

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Archibald, L. K., Dobbie, H., Kazembe, P., Nwanyanwu, O., McKnight, C., Byrne, T., … Jarvis, W. R. (2001). Utility of paired BACTEC MYCO/F LYTIC blood culture vials for detection of bacteremia, mycobacteremia, and fungemia. J Clin Microbiol, 39(5), 1960–1962. https://doi.org/10.1128/JCM.39.5.1960-1962.2001
Archibald, L. K., H. Dobbie, P. Kazembe, O. Nwanyanwu, C. McKnight, T. Byrne, R. M. Addison, M. Bell, L. B. Reller, and W. R. Jarvis. “Utility of paired BACTEC MYCO/F LYTIC blood culture vials for detection of bacteremia, mycobacteremia, and fungemia.J Clin Microbiol 39, no. 5 (May 2001): 1960–62. https://doi.org/10.1128/JCM.39.5.1960-1962.2001.
Archibald LK, Dobbie H, Kazembe P, Nwanyanwu O, McKnight C, Byrne T, et al. Utility of paired BACTEC MYCO/F LYTIC blood culture vials for detection of bacteremia, mycobacteremia, and fungemia. J Clin Microbiol. 2001 May;39(5):1960–2.
Archibald, L. K., et al. “Utility of paired BACTEC MYCO/F LYTIC blood culture vials for detection of bacteremia, mycobacteremia, and fungemia.J Clin Microbiol, vol. 39, no. 5, May 2001, pp. 1960–62. Pubmed, doi:10.1128/JCM.39.5.1960-1962.2001.
Archibald LK, Dobbie H, Kazembe P, Nwanyanwu O, McKnight C, Byrne T, Addison RM, Bell M, Reller LB, Jarvis WR. Utility of paired BACTEC MYCO/F LYTIC blood culture vials for detection of bacteremia, mycobacteremia, and fungemia. J Clin Microbiol. 2001 May;39(5):1960–1962.

Published In

J Clin Microbiol

DOI

ISSN

0095-1137

Publication Date

May 2001

Volume

39

Issue

5

Start / End Page

1960 / 1962

Location

United States

Related Subject Headings

  • Sensitivity and Specificity
  • Reagent Kits, Diagnostic
  • Mycobacterium Infections
  • Mycobacterium
  • Microbiology
  • Humans
  • Fungemia
  • Culture Media
  • Blood
  • Bacteremia