Skip to main content
Journal cover image

The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1.

Publication ,  Journal Article
Goldbaum, FA; Schwarz, FP; Eisenstein, E; Cauerhff, A; Mariuzza, RA; Poljak, RJ
Published in: Journal of molecular recognition : JMR
1996

The reactions of lysozyme with the specific monoclonal antibody D1.3, its Fv fragment and a mutant of the Fv, were studied under conditions of reduced water activity through the addition of the cosolutes glycerol, ethanol, dioxane and methanol. Titration calorimetry, BIAcoreTM and ultracentrifugal analyses were used to determine enthalpy of reactions and affinity constants. There was a decrease in the values of the enthalpies of reactions as well as in the association constants which was proportional to the decrease in water activity. These results are consistent with a structural model in which water molecules bound to the antigen and the antibody are conserved upon complex formation and provide bonds which are important for the stability of the complex. In contrast, the reaction of lysozyme with the specific monoclonal antibody D44.1, or its Fab, showed the inverse effect: a small increase in the value of the association constant with decreasing water molarities. This is in agreement with a model in which binding of antigen to antibody D44.1 is accompanied by the release of a very small number of water molecules.

Duke Scholars

Published In

Journal of molecular recognition : JMR

ISSN

0952-3499

Publication Date

1996

Volume

9

Issue

1

Start / End Page

6 / 12

Location

england

Related Subject Headings

  • Biophysics
  • 03 Chemical Sciences
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Goldbaum, F. A., Schwarz, F. P., Eisenstein, E., Cauerhff, A., Mariuzza, R. A., & Poljak, R. J. (1996). The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1. Journal of Molecular Recognition : JMR, 9(1), 6–12.
Goldbaum, F. A., F. P. Schwarz, E. Eisenstein, A. Cauerhff, R. A. Mariuzza, and R. J. Poljak. “The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1.Journal of Molecular Recognition : JMR 9, no. 1 (1996): 6–12.
Goldbaum FA, Schwarz FP, Eisenstein E, Cauerhff A, Mariuzza RA, Poljak RJ. The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1. Journal of molecular recognition : JMR. 1996;9(1):6–12.
Goldbaum, F. A., et al. “The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1.Journal of Molecular Recognition : JMR, vol. 9, no. 1, 1996, pp. 6–12.
Goldbaum FA, Schwarz FP, Eisenstein E, Cauerhff A, Mariuzza RA, Poljak RJ. The effect of water activity on the association constant and the enthalpy of reaction between lysozyme and the specific antibodies D1.3 and D44.1. Journal of molecular recognition : JMR. 1996;9(1):6–12.
Journal cover image

Published In

Journal of molecular recognition : JMR

ISSN

0952-3499

Publication Date

1996

Volume

9

Issue

1

Start / End Page

6 / 12

Location

england

Related Subject Headings

  • Biophysics
  • 03 Chemical Sciences