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Structural and physicochemical analysis of the reaction between the anti-lysozyme antibody D1.3 and the anti-idiotopic antibodies E225 and E5.2.

Publication ,  Journal Article
Tello, D; Eisenstein, E; Schwarz, FP; Goldbaum, FA; Fields, BA; Mariuzza, RA; Poljak, RJ
Published in: Journal of molecular recognition : JMR
March 1994

The reaction between the mouse (BALB/c) anti-idiotopic monoclonal antibodies E225 and E5.2 and idiotopes on the (BALB/c) anti-lysozyme monoclonal antibody D1.3 has been characterized by titration calorimetry, by equilibrium sedimentation and by the determination of binding association and dissociation rates. The reaction between E5.2 and D1.3 is driven by a large negative enthalpy and its rate and equilibrium association constants are comparable to those observed in other antigen-antibody reactions. In contrast, the reaction between E225 and D1.3 is entropically driven and characterized by slow association kinetics (1 x 10(3) M-1 sec-1) and a resulting low equilibrium constant (Ka = 2 x 10(5) M-1). A correlation of these properties with the three-dimensional structure of the Fab225-FabD1.3 complex, previously determined by X-ray diffraction methods to 2.5 A resolution, indicates that conformational changes of several D1.3 contacting residues, located in its complementarity determining regions, may explain these features of the reaction.

Duke Scholars

Published In

Journal of molecular recognition : JMR

ISSN

0952-3499

Publication Date

March 1994

Volume

7

Issue

1

Start / End Page

57 / 62

Location

england

Related Subject Headings

  • Biophysics
  • 03 Chemical Sciences
 

Citation

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ICMJE
MLA
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Tello, D., Eisenstein, E., Schwarz, F. P., Goldbaum, F. A., Fields, B. A., Mariuzza, R. A., & Poljak, R. J. (1994). Structural and physicochemical analysis of the reaction between the anti-lysozyme antibody D1.3 and the anti-idiotopic antibodies E225 and E5.2. Journal of Molecular Recognition : JMR, 7(1), 57–62.
Tello, D., E. Eisenstein, F. P. Schwarz, F. A. Goldbaum, B. A. Fields, R. A. Mariuzza, and R. J. Poljak. “Structural and physicochemical analysis of the reaction between the anti-lysozyme antibody D1.3 and the anti-idiotopic antibodies E225 and E5.2.Journal of Molecular Recognition : JMR 7, no. 1 (March 1994): 57–62.
Tello D, Eisenstein E, Schwarz FP, Goldbaum FA, Fields BA, Mariuzza RA, et al. Structural and physicochemical analysis of the reaction between the anti-lysozyme antibody D1.3 and the anti-idiotopic antibodies E225 and E5.2. Journal of molecular recognition : JMR. 1994 Mar;7(1):57–62.
Tello, D., et al. “Structural and physicochemical analysis of the reaction between the anti-lysozyme antibody D1.3 and the anti-idiotopic antibodies E225 and E5.2.Journal of Molecular Recognition : JMR, vol. 7, no. 1, Mar. 1994, pp. 57–62.
Tello D, Eisenstein E, Schwarz FP, Goldbaum FA, Fields BA, Mariuzza RA, Poljak RJ. Structural and physicochemical analysis of the reaction between the anti-lysozyme antibody D1.3 and the anti-idiotopic antibodies E225 and E5.2. Journal of molecular recognition : JMR. 1994 Mar;7(1):57–62.
Journal cover image

Published In

Journal of molecular recognition : JMR

ISSN

0952-3499

Publication Date

March 1994

Volume

7

Issue

1

Start / End Page

57 / 62

Location

england

Related Subject Headings

  • Biophysics
  • 03 Chemical Sciences