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Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition.

Publication ,  Journal Article
Schinkel, JE; Hammes, GG
Published in: Biochemistry
July 15, 1986

Time-resolved fluorescence depolarization measurements were made on chloroplast coupling factor 1 (CF1) labeled with pyrenylmaleimide. Rotational correlation times were determined for native CF1, for CF1 lacking epsilon and/or delta polypeptides, and for activated enzyme. The rotational correlation time measured is characteristic of the rotation of the entire enzyme. Removal of the delta polypeptide resulted in a 25% smaller rotational correlation time, although the delta polypeptide contributes less than 5% of the mass of CF1. Removal of the epsilon polypeptide was without effect. Simultaneous removal of delta and epsilon polypeptides produced a 30% smaller rotational correlation time. Activation of CF1 ATPase by incubation with dithiothreitol reduced the rotational correlation time by 15% relative to that of the latent enzyme. The rotational correlation time of CF1 with delta and epsilon polypeptides removed is essentially that expected for a spherical molecule, whereas the other forms of the enzyme can be approximated as ellipsoids of revolution; the axial ratio of the latent enzyme is estimated from the rotational correlation time and the intrinsic viscosity. These data indicate that the delta polypeptide significantly alters the shape of the enzyme and that a conformational change accompanies dithiothreitol activation of the enzyme.

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

July 15, 1986

Volume

25

Issue

14

Start / End Page

4066 / 4071

Location

United States

Related Subject Headings

  • Time Factors
  • Spectrophotometry
  • Spectrometry, Fluorescence
  • Proton-Translocating ATPases
  • Protein Conformation
  • Plants
  • Molecular Weight
  • Macromolecular Substances
  • Kinetics
  • Calcium-Transporting ATPases
 

Citation

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Schinkel, J. E., & Hammes, G. G. (1986). Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition. Biochemistry, 25(14), 4066–4071. https://doi.org/10.1021/bi00362a012
Schinkel, J. E., and G. G. Hammes. “Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition.Biochemistry 25, no. 14 (July 15, 1986): 4066–71. https://doi.org/10.1021/bi00362a012.
Schinkel JE, Hammes GG. Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition. Biochemistry. 1986 Jul 15;25(14):4066–71.
Schinkel, J. E., and G. G. Hammes. “Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition.Biochemistry, vol. 25, no. 14, July 1986, pp. 4066–71. Pubmed, doi:10.1021/bi00362a012.
Schinkel JE, Hammes GG. Chloroplast coupling factor 1: dependence of rotational correlation time on polypeptide composition. Biochemistry. 1986 Jul 15;25(14):4066–4071.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

July 15, 1986

Volume

25

Issue

14

Start / End Page

4066 / 4071

Location

United States

Related Subject Headings

  • Time Factors
  • Spectrophotometry
  • Spectrometry, Fluorescence
  • Proton-Translocating ATPases
  • Protein Conformation
  • Plants
  • Molecular Weight
  • Macromolecular Substances
  • Kinetics
  • Calcium-Transporting ATPases