Skip to main content
Journal cover image

Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.

Publication ,  Journal Article
Admon, A; Hammes, GG
Published in: Biochemistry
June 2, 1987

The labeling of chloroplast coupling factor 1 by 3'-O-(4-benzoyl)benzoyl-ATP (BzATP) was studied. When the enzyme was incubated with approximately 10 microM BzATP and 6 mM MgCl2 at pH 7.9 for approximately 20 min and passed through two Sephadex G-50 centrifuge columns, three BzATP molecules were bound per coupling factor molecule. Photolysis of radioactive enzyme-bound BzATP followed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and autoradiography revealed that the BzATP bound primarily to the beta-polypeptide. If unbound BzATP was not removed by centrifuge columns prior to photolysis, significant labeling of the alpha-polypeptide also occurred. After photolysis, the BzATP-labeled enzyme was treated with trypsin, and two radioactive peptides were isolated by high-performance liquid chromatography on a C18 column. The two peptides were sequenced and found to correspond to amino acids 360-378 and 393-397 of the beta-polypeptide. For the sequence 360-378, two specific amino acids were found to be radioactive (Tyr-362 and Asp-369). This region of the polypeptide is highly conserved in several different species and probably corresponds to part of the nucleotide binding region of the catalytic site. In the case of amino acids 393-397, a very low level of radioactivity was found for all amino acids. The significance of this peptide for the binding of nucleotides to coupling factor 1 could not be established.

Duke Scholars

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

June 2, 1987

Volume

26

Issue

11

Start / End Page

3193 / 3197

Location

United States

Related Subject Headings

  • Proton-Translocating ATPases
  • Protein Binding
  • Plants
  • Kinetics
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Amino Acid Sequence
  • Affinity Labels
  • Adenosine Triphosphate
  • 1101 Medical Biochemistry and Metabolomics
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Admon, A., & Hammes, G. G. (1987). Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1. Biochemistry, 26(11), 3193–3197. https://doi.org/10.1021/bi00385a038
Admon, A., and G. G. Hammes. “Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.Biochemistry 26, no. 11 (June 2, 1987): 3193–97. https://doi.org/10.1021/bi00385a038.
Admon A, Hammes GG. Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1. Biochemistry. 1987 Jun 2;26(11):3193–7.
Admon, A., and G. G. Hammes. “Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1.Biochemistry, vol. 26, no. 11, June 1987, pp. 3193–97. Pubmed, doi:10.1021/bi00385a038.
Admon A, Hammes GG. Amino acid sequence of the nucleotide binding region of chloroplast coupling factor 1. Biochemistry. 1987 Jun 2;26(11):3193–3197.
Journal cover image

Published In

Biochemistry

DOI

ISSN

0006-2960

Publication Date

June 2, 1987

Volume

26

Issue

11

Start / End Page

3193 / 3197

Location

United States

Related Subject Headings

  • Proton-Translocating ATPases
  • Protein Binding
  • Plants
  • Kinetics
  • Biochemistry & Molecular Biology
  • Binding Sites
  • Amino Acid Sequence
  • Affinity Labels
  • Adenosine Triphosphate
  • 1101 Medical Biochemistry and Metabolomics