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The dishevelled protein is modified by wingless signaling in Drosophila.

Publication ,  Journal Article
Yanagawa, S; van Leeuwen, F; Wodarz, A; Klingensmith, J; Nusse, R
Published in: Genes Dev
May 1, 1995

Wingless (Wg) is an important signaling molecule in the development of Drosophila, but little is known about its signal transduction pathway. Genetic evidence indicates that another segment polarity gene, dishevelled (dsh) is required for Wg signaling. We have recently developed a cell culture system for Wg protein activity, and using this in vitro system as well as intact Drosophila embryos, we have analyzed biochemical changes in the Dsh protein as a consequence of Wg signaling. We find that Dsh is a phosphoprotein, normally present in the cytoplasm. Wg signaling generates a hyperphosphorylated form of Dsh, which is associated with a membrane fraction. Overexpressed Dsh becomes hyperphosphorylated in the absence of extracellular Wg and increases levels of the Armadillo protein, thereby mimicking the Wg signal. A deletional analysis of Dsh identifies several conserved domains essential for activity, among which is a so-called GLGF/DHR motif. We conclude that dsh, a highly conserved gene, is not merely a permissive factor in Wg signaling but encodes a novel signal transduction molecule, which may function between the Wg receptor and more downstream signaling molecules.

Duke Scholars

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Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

May 1, 1995

Volume

9

Issue

9

Start / End Page

1087 / 1097

Location

United States

Related Subject Headings

  • Wnt1 Protein
  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins
  • Proteins
  • Protein Biosynthesis
  • Phosphoproteins
 

Citation

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Yanagawa, S., van Leeuwen, F., Wodarz, A., Klingensmith, J., & Nusse, R. (1995). The dishevelled protein is modified by wingless signaling in Drosophila. Genes Dev, 9(9), 1087–1097. https://doi.org/10.1101/gad.9.9.1087
Yanagawa, S., F. van Leeuwen, A. Wodarz, J. Klingensmith, and R. Nusse. “The dishevelled protein is modified by wingless signaling in Drosophila.Genes Dev 9, no. 9 (May 1, 1995): 1087–97. https://doi.org/10.1101/gad.9.9.1087.
Yanagawa S, van Leeuwen F, Wodarz A, Klingensmith J, Nusse R. The dishevelled protein is modified by wingless signaling in Drosophila. Genes Dev. 1995 May 1;9(9):1087–97.
Yanagawa, S., et al. “The dishevelled protein is modified by wingless signaling in Drosophila.Genes Dev, vol. 9, no. 9, May 1995, pp. 1087–97. Pubmed, doi:10.1101/gad.9.9.1087.
Yanagawa S, van Leeuwen F, Wodarz A, Klingensmith J, Nusse R. The dishevelled protein is modified by wingless signaling in Drosophila. Genes Dev. 1995 May 1;9(9):1087–1097.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

May 1, 1995

Volume

9

Issue

9

Start / End Page

1087 / 1097

Location

United States

Related Subject Headings

  • Wnt1 Protein
  • Transcription Factors
  • Trans-Activators
  • Signal Transduction
  • Sequence Deletion
  • Recombinant Fusion Proteins
  • Proto-Oncogene Proteins
  • Proteins
  • Protein Biosynthesis
  • Phosphoproteins